Anti-Vimentin/VIM Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC IF

PB9359

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-Vimentin antibody (PB9359). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human U20S whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: human Hela whole cell lysates,
Lane 6: human THP-1 whole cell lysates,
Lane 7: human A549 whole cell lysates,
Lane 8: human MCF-7 whole cell lysates,
Lane 9: rat ovary tissue lysates,
Lane 10: rat lung tissue lysates,
Lane 11: mouse lung tissue lysates,
Lane 12: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Vimentin antigen affinity purified polyclonal antibody (PB9359) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Vimentin at approximately 56 kDa. The expected band size for Vimentin is at 54 kDa.
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IHC analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of mouse cardiac muscle tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(12)
IHC analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of rat cardiac muscle tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(12)
IHC analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(12)
IHC analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of human placenta tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(12)
IHC analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(12)
IHC analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(12)
IHC analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of human invasive urothelial carcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(12)
IHC analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(12)
IHC analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(12)
IF analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of mouse brain tissue. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(12)
IF analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of rat brain tissue. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(12)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Vimentin/VIM Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

VIM; vimentin

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IF

基因名称

VIM

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Vimentin, identical to the related mouse and rat sequences.

计算分子量

54 kDa

实际分子量

54 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

VIM(vimentin) is also known as HEL113 or CTRCT30. This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.

Uniprot ID

P08670

RRID

AB_3082084

文献引用格式

Vimentin/VIM Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9359)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

vimentin

蛋白名全称

Vimentin

实验方案>

实验步骤

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