Western blot analysis of anti-Vimentin antibody (BM0135). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A375 whole cell lysates,
Lane 3: human U87 whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Vimentin antigen affinity purified monoclonal antibody (BM0135) and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Vimentin at approximately 56 kDa. The expected band size for Vimentin is at 54 kDa.
IHC analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of human placenta tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of human invasive urothelial carcinoma tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of human invasive urothelial carcinoma tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of rat brain tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of human intestinal cancer tissue. Fluoro594-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog # BA1141) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:1000-5000 |
| Immunofluorescence (IF): | 1:1000-5000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of anti-Vimentin antibody (BM0135). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A375 whole cell lysates,
Lane 3: human U87 whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Vimentin antigen affinity purified monoclonal antibody (BM0135) and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Vimentin at approximately 56 kDa. The expected band size for Vimentin is at 54 kDa.
IHC analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of human placenta tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of human invasive urothelial carcinoma tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of human invasive urothelial carcinoma tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of rat brain tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of human intestinal cancer tissue. Fluoro594-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog # BA1141) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Western blot analysis of anti-Vimentin antibody (BM0135). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A375 whole cell lysates,
Lane 3: human U87 whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Vimentin antigen affinity purified monoclonal antibody (BM0135) and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Vimentin at approximately 56 kDa. The expected band size for Vimentin is at 54 kDa.
IHC analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of human placenta tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of human invasive urothelial carcinoma tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of human invasive urothelial carcinoma tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of rat brain tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of Vimentin using anti-Vimentin antibody (BM0135).
Vimentin was detected in a paraffin-embedded section of human intestinal cancer tissue. Fluoro594-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog # BA1141) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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