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Anti-JNK2/MAPK9 Antibody (Clone#EIF-13)

Rabbit monoclonal antibody

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筛选器: All WB IHC ICC/IF IP FCM

BM4462

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of anti-JNK2/MAPK9 antibody (BM4462). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Hela whole cell lysates,
    Lane 2: human 293T whole cell lysates,
    Lane 3: monkey COS-7 whole cell lysates,
    Lane 4: rat brain tissue lysates,
    Lane 5: rat liver tissue lysates,
    Lane 6: mouse brain tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-JNK2/MAPK9 antigen affinity purified monoclonal antibody (BM4462) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for JNK2/MAPK9 at approximately 48 kDa. The expected band size for JNK2/MAPK9 is at 48 kDa.

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产品名称
Anti-JNK2/MAPK9 Antibody (Clone#EIF-13)
规格/价格
50μl|1280 100μl|2180 150μl|2800
指标别称
c Jun N terminal kinase 2; JNK 55; JNK2; JNK2A; JNK2ALPHA; JNK2B; JNK2BETA; MAP kinase 9; MAPK 9; MAPK9; p54a; p54aSAPK; PRKM9; SAPK
产品类型
Monoclonal
检验物种
human, mouse, rat, monkey
应用范围
WB, IHC, ICC/IF, IP, FCM
基因名称
MAPK9
克隆号
EIF-13
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthesized peptide derived from human JNK2
计算分子量
48 kDa
实际分子量
48 kDa
成分
500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.
纯化方式
Affinity-chromatography
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
JNK2 is also known as MAPK9. The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. Also, this gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.
Uniprot ID
P45984  
文献引用格式
JNK2/MAPK9 Antibody (Clone#EIF-13) (Boster Biological Technology, Wuhan, China. Catalog#BM4462)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
Mitogen-activated protein kinase 9
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-200
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-200
ImmunoPrecipitation (IP):1:20
Flow Cytometry (FCM):1:20

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    Anti-JNK2/MAPK9 Antibody (Clone#EIF-13)

    筛选器: All WB IHC ICC/IF IP FCM

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