Anti-JNK2/MAPK9 Antibody

筛选器： All WB ICC/IF FCM

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  Western blot analysis of JNK2/MAPK9 using anti-JNK2/MAPK9 antibody (PB0522). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human SiHa whole cell lysates,
  Lane 2: human U251 whole cell lysates,
  Lane 3: human Caco-2 whole cell lysates,
  Lane 4: human A549 whole cell lysates,
  Lane 5: rat brain tissue lysates,
  Lane 6: rat C6 whole cell lysates,
  Lane 7: mouse brain tissue lysates,
  Lane 8: mouse Neuro-2a whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-JNK2/MAPK9 antigen affinity purified polyclonal antibody (PB0522) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for JNK2/MAPK9 at approximately 48-50 kDa. The expected band size for JNK2/MAPK9 is at 48 kDa.

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  ICC/IF analysis of JNK2/MAPK9 using anti-JNK2/MAPK9 antibody (PB0522).
  JNK2/MAPK9 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-JNK2/MAPK9 Antibody (PB0522) at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Flow Cytometry analysis of HEL cells using anti-JNK2/MAPK9 antibody (PB0522).
  Overlay histogram showing HEL cells stained with PB0522 (Blue line). To facilitate intrMyelin basic protein/MBPllular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JNK2/MAPK9 Antibody (PB0522) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Flow Cytometry analysis of Hepa1-6 cells using anti-JNK2/MAPK9 antibody (PB0522).
  Overlay histogram showing Hepa1-6 cells stained with PB0522 (Blue line). To facilitate intrMyelin basic protein/MBPllular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JNK2/MAPK9 Antibody (PB0522) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Flow Cytometry analysis of RH-35 cells using anti-JNK2/MAPK9 antibody (PB0522).
  Overlay histogram showing RH-35 cells stained with PB0522 (Blue line). To facilitate intrMyelin basic protein/MBPllular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JNK2/MAPK9 Antibody (PB0522) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of JNK2/MAPK9 using anti-JNK2/MAPK9 antibody (PB0522). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human SiHa whole cell lysates,
  Lane 2: human U251 whole cell lysates,
  Lane 3: human Caco-2 whole cell lysates,
  Lane 4: human A549 whole cell lysates,
  Lane 5: rat brain tissue lysates,
  Lane 6: rat C6 whole cell lysates,
  Lane 7: mouse brain tissue lysates,
  Lane 8: mouse Neuro-2a whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-JNK2/MAPK9 antigen affinity purified polyclonal antibody (PB0522) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for JNK2/MAPK9 at approximately 48-50 kDa. The expected band size for JNK2/MAPK9 is at 48 kDa.

• 

  ICC/IF analysis of JNK2/MAPK9 using anti-JNK2/MAPK9 antibody (PB0522).
  JNK2/MAPK9 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-JNK2/MAPK9 Antibody (PB0522) at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Flow Cytometry analysis of HEL cells using anti-JNK2/MAPK9 antibody (PB0522).
  Overlay histogram showing HEL cells stained with PB0522 (Blue line). To facilitate intrMyelin basic protein/MBPllular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JNK2/MAPK9 Antibody (PB0522) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Flow Cytometry analysis of Hepa1-6 cells using anti-JNK2/MAPK9 antibody (PB0522).
  Overlay histogram showing Hepa1-6 cells stained with PB0522 (Blue line). To facilitate intrMyelin basic protein/MBPllular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JNK2/MAPK9 Antibody (PB0522) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Flow Cytometry analysis of RH-35 cells using anti-JNK2/MAPK9 antibody (PB0522).
  Overlay histogram showing RH-35 cells stained with PB0522 (Blue line). To facilitate intrMyelin basic protein/MBPllular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JNK2/MAPK9 Antibody (PB0522) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.