Anti-ER/ESR1 (Phospho-S118) Antibody (Clone#EIG-5)

Rabbit Monoclonal antibody

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筛选器： All WB IHC ICC/IF

BM4463

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of anti-ER/ESR1 (Phospho-S118) antibody (BM4463). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates,
Lane 3: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ER/ESR1 (Phospho-S118) antigen affinity purified monoclonal antibody (BM4463) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ER/ESR1 (Phospho-S118) at approximately 66 kDa. The expected band size for ER/ESR1 (Phospho-S118) is at 66 kDa.
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IHC analysis of p-ESR using anti-p-ESR antibody (BM4463) .
p-ESR was detected in a paraffin-embedded section of rat uterus tissue. The tissue section was incubated with rabbit anti-p-ESR Antibody (BM4463) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of p-ESR using anti-p-ESR antibody (BM4463) .
p-ESR was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-p-ESR Antibody (BM4463) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of p-ESR using anti-p-ESR antibody (BM4463) .
p-ESR was detected in a paraffin-embedded section of human prostatic hyperplasia tissue. The tissue section was incubated with rabbit anti-p-ESR Antibody (BM4463) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
Immunofluorescent analysis of MCF7 cells treated with EGF, using Phospho-ER alpha (S118) Antibody.
all(5)

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产品简介>

产品名称

Anti-ER/ESR1 (Phospho-S118) Antibody (Clone#EIG-5)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ER; ER alpha; Era; ERα; ESR; ESR1; ESRA; Estradiol receptor; Estrogen receptor; estrogen receptor 1; NR3A1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

ESR1

克隆号

EIG-5

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human ER alpha

计算分子量

66 kDa

实际分子量

66 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Estrogen receptor alpha (ER-α), also known as NR3A1, is one of two main types of estrogen receptor, a nuclear receptor that is activated by the sex hormone estrogen. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. In humans, ER-α is encoded by the gene ESR1 (Estrogen Receptor 1). It is mapped to 6q25.1. This gene is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, it also play a role in other tissues such as bone.

Uniprot ID

P03372

文献引用格式

ER/ESR1 (Phospho-S118) Antibody (Clone#EIG-5) (Boster Biological Technology, Wuhan, China. Catalog#BM4463)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Estrogen receptor

实验方案>

实验步骤

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