Anti-ER/ESR1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

PB0188

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of anti-ER/ESR1 antibody (PB0188). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human T-47D whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ER/ESR1 antigen affinity purified polyclonal antibody (PB0188) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ER/ESR1 at approximately 66 kDa. The expected band size for ER/ESR1 is at 66 kDa.
all(2)
Figure 2. IHC analysis of ER/ESR1 using anti-ER/ESR1 antibody (PB0188).
ER/ESR1 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ER/ESR1 Antibody (PB0188) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(2)

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产品简介>

产品名称

Anti-ER/ESR1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ER; ER alpha; Era; ERα; ESR; ESR1; ESRA; Estradiol receptor; Estrogen receptor; estrogen receptor 1; NR3A1

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC

基因名称

ESR1

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human Estrogen Receptor recombinant protein (Position: F425-V595). Human Estrogen Receptor shares 89% and 88% amino acid (aa) sequences identity with mouse and rat Estrogen Receptor, respectively.

计算分子量

66 kDa

实际分子量

66 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Estrogen receptor alpha (ER-α), also known as NR3A1, is one of two main types of estrogen receptor, a nuclear receptor that is activated by the sex hormone estrogen. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. In humans, ER-α is encoded by the gene ESR1 (Estrogen Receptor 1). It is mapped to 6q25.1. This gene is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, it also play a role in other tissues such as bone.

Uniprot ID

P03372

文献引用格式

ER/ESR1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0188)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

estrogen receptor 1

蛋白名全称

Estrogen receptor

实验方案>

实验步骤

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