Anti-Alix/PDCD6IP Antibody (Clone#AEBO-16)

Rabbit monoclonal antibody

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筛选器： All WB FCM

BM5496

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-Alix/PDCD6IP antibody (BM5496). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human Daudi whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: rat lung tissue lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Alix/PDCD6IP antigen affinity purified monoclonal antibody (BM5496) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Alix/PDCD6IP at approximately 96 kDa. The expected band size for Alix/PDCD6IP is at 96 kDa.
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产品简介>

产品名称

Anti-Alix/PDCD6IP Antibody (Clone#AEBO-16)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

AIP1; ALG 2 interacting protein 1; Alix; DRIP4; HP95; KIAA1375; PDCD6 interacting protein; PDCD6IP

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, FCM

基因名称

PDCD6IP

克隆号

AEBO-16

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human ALIX Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome.

计算分子量

96 kDa

实际分子量

96 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Programmed cell death 6-interacting protein is a protein that in humans is encoded by the PDCD6IP gene. This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Uniprot ID

Q8WUM4

文献引用格式

Alix/PDCD6IP Antibody (Clone#AEBO-16) (Boster Biological Technology, Wuhan, China. Catalog#BM5496)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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