Western blot analysis of Alix/PDCD6IP using anti-Alix/PDCD6IP antibody (M01751-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human PANC-1 whole cell lysates,
Lane 5: human K562 whole cell lysates,
Lane 6: human SW579 whole cell lysates,
Lane 7: rat RH35 whole cell lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Alix/PDCD6IP antigen affinity purified monoclonal antibody (M01751-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Alix/PDCD6IP at approximately 96 kDa. The expected band size for Alix/PDCD6IP is at 96 kDa.
IHC analysis of Alix/PDCD6IP using anti-Alix/PDCD6IP antibody (M01751-1).
Alix/PDCD6IP was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Alix/PDCD6IP Antibody (M01751-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Alix/PDCD6IP using anti-Alix/PDCD6IP antibody (M01751-1).
Alix/PDCD6IP was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Alix/PDCD6IP Antibody (M01751-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Alix/PDCD6IP using anti-Alix/PDCD6IP antibody (M01751-1).
Alix/PDCD6IP was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Alix/PDCD6IP Antibody (M01751-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Alix/PDCD6IP using anti-Alix/PDCD6IP antibody (M01751-1).
Alix/PDCD6IP was detected in a paraffin-embedded section of mouse testis tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Alix/PDCD6IP Antibody (M01751-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Alix/PDCD6IP using anti-Alix/PDCD6IP antibody (M01751-1).
Alix/PDCD6IP was detected in a paraffin-embedded section of rat testis tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Alix/PDCD6IP Antibody (M01751-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Alix/PDCD6IP using anti-Alix/PDCD6IP antibody (M01751-1).
Alix/PDCD6IP was detected in a paraffin-embedded section of rat testis tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Alix/PDCD6IP Antibody (M01751-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of PDCD6IP using anti- PDCD6IP antibody (M01751-1).
PDCD6IP was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti- PDCD6IP Antibody (M01751-1) overnight at 4°C. DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Flow Cytometry analysis of A431 cells using anti-Alix/PDCD6IP antibody (M01751-1).
Overlay histogram showing A431 cells stained with M01751-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Alix/PDCD6IP Antibody (M01751-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of Alix/PDCD6IP using anti-Alix/PDCD6IP antibody (M01751-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human PANC-1 whole cell lysates,
Lane 5: human K562 whole cell lysates,
Lane 6: human SW579 whole cell lysates,
Lane 7: rat RH35 whole cell lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Alix/PDCD6IP antigen affinity purified monoclonal antibody (M01751-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Alix/PDCD6IP at approximately 96 kDa. The expected band size for Alix/PDCD6IP is at 96 kDa.
IHC analysis of Alix/PDCD6IP using anti-Alix/PDCD6IP antibody (M01751-1).
Alix/PDCD6IP was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Alix/PDCD6IP Antibody (M01751-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Alix/PDCD6IP using anti-Alix/PDCD6IP antibody (M01751-1).
Alix/PDCD6IP was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Alix/PDCD6IP Antibody (M01751-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Alix/PDCD6IP using anti-Alix/PDCD6IP antibody (M01751-1).
Alix/PDCD6IP was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Alix/PDCD6IP Antibody (M01751-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Alix/PDCD6IP using anti-Alix/PDCD6IP antibody (M01751-1).
Alix/PDCD6IP was detected in a paraffin-embedded section of mouse testis tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Alix/PDCD6IP Antibody (M01751-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Alix/PDCD6IP using anti-Alix/PDCD6IP antibody (M01751-1).
Alix/PDCD6IP was detected in a paraffin-embedded section of rat testis tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Alix/PDCD6IP Antibody (M01751-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Alix/PDCD6IP using anti-Alix/PDCD6IP antibody (M01751-1).
Alix/PDCD6IP was detected in a paraffin-embedded section of rat testis tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Alix/PDCD6IP Antibody (M01751-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of PDCD6IP using anti- PDCD6IP antibody (M01751-1).
PDCD6IP was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti- PDCD6IP Antibody (M01751-1) overnight at 4°C. DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Flow Cytometry analysis of A431 cells using anti-Alix/PDCD6IP antibody (M01751-1).
Overlay histogram showing A431 cells stained with M01751-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Alix/PDCD6IP Antibody (M01751-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Western blot analysis of Alix/PDCD6IP using anti-Alix/PDCD6IP antibody (M01751-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human PANC-1 whole cell lysates,
Lane 5: human K562 whole cell lysates,
Lane 6: human SW579 whole cell lysates,
Lane 7: rat RH35 whole cell lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Alix/PDCD6IP antigen affinity purified monoclonal antibody (M01751-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Alix/PDCD6IP at approximately 96 kDa. The expected band size for Alix/PDCD6IP is at 96 kDa.
IHC analysis of Alix/PDCD6IP using anti-Alix/PDCD6IP antibody (M01751-1).
Alix/PDCD6IP was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Alix/PDCD6IP Antibody (M01751-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Alix/PDCD6IP using anti-Alix/PDCD6IP antibody (M01751-1).
Alix/PDCD6IP was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Alix/PDCD6IP Antibody (M01751-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Alix/PDCD6IP using anti-Alix/PDCD6IP antibody (M01751-1).
Alix/PDCD6IP was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Alix/PDCD6IP Antibody (M01751-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Alix/PDCD6IP using anti-Alix/PDCD6IP antibody (M01751-1).
Alix/PDCD6IP was detected in a paraffin-embedded section of mouse testis tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Alix/PDCD6IP Antibody (M01751-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Alix/PDCD6IP using anti-Alix/PDCD6IP antibody (M01751-1).
Alix/PDCD6IP was detected in a paraffin-embedded section of rat testis tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Alix/PDCD6IP Antibody (M01751-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Alix/PDCD6IP using anti-Alix/PDCD6IP antibody (M01751-1).
Alix/PDCD6IP was detected in a paraffin-embedded section of rat testis tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Alix/PDCD6IP Antibody (M01751-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of PDCD6IP using anti- PDCD6IP antibody (M01751-1).
PDCD6IP was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti- PDCD6IP Antibody (M01751-1) overnight at 4°C. DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Flow Cytometry analysis of A431 cells using anti-Alix/PDCD6IP antibody (M01751-1).
Overlay histogram showing A431 cells stained with M01751-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Alix/PDCD6IP Antibody (M01751-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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