Anti-Alix/PDCD6IP Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC IF ICC/IF FCM

PB0799

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Alix/PDCD6IP using anti-Alix/PDCD6IP antibody (PB0799). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: rat liver tissue lysates,
Lane 4: rat RH35 whole cell lysates,
Lane 5: mouse liver tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Alix/PDCD6IP antigA03957-Aen affinity purified polyclonal antibody (PB0799) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Alix/PDCD6IP at approximately 96 kDa. The expected band size for Alix/PDCD6IP is at 96 kDa.
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IHC analysis of Alix/PDCD6IP using anti-Alix/PDCD6IP antibody (PB0799).
Alix/PDCD6IP was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with rabbit anti-Alix/PDCD6IP Antibody (PB0799) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis of Alix/PDCD6IP using anti-Alix/PDCD6IP antibody (PB0799).
Alix/PDCD6IP was detected in a paraffin-embedded section of human pancreatic cancer tissue. The tissue section was incubated with rabbit anti-Alix/PDCD6IP Antibody (PB0799) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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IF analysis of Alix/PDCD6IP using anti-Alix/PDCD6IP antibody (PB0799).
Alix/PDCD6IP was detected in a paraffin-embedded section of human prostate cancer tissue. The tissue section was incubated with rabbit anti-Alix/PDCD6IP Antibody (PB0799) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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IF analysis of Alix/PDCD6IP using anti-Alix/PDCD6IP antibody (PB0799).
Alix/PDCD6IP was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-Alix/PDCD6IP Antibody (PB0799) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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Flow Cytometry analysis of Jurkat cells using anti-Alix/PDCD6IP antibody (PB0799).
Overlay histogram showing Jurkat cells stained with PB0799 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Alix/PDCD6IP Antibody (PB0799) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Alix/PDCD6IP Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

AIP1; ALG 2 interacting protein 1; Alix; DRIP4; HP95; KIAA1375; PDCD6 interacting protein; PDCD6IP

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IF, ICC/IF, FCM

基因名称

PDCD6IP

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human ALIX recombinant protein (Position: A2-D330). Human ALIX shares 96.7% and 95.2% amino acid (aa) sequence identity with mouse and rat ALIX, respectively.

计算分子量

96 kDa

实际分子量

96 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Programmed cell death 6-interacting protein is a protein that in humans is encoded by the PDCD6IP gene. This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Uniprot ID

Q8WUM4

文献引用格式

Alix/PDCD6IP Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0799)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

programmed cell death 6 interacting protein

蛋白名全称

Programmed cell death 6-interacting protein

实验方案>

实验步骤

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