Human IGFBP3 ELISA Kit

96T|2480

BP 53; IBP 3; IBP3; IGF binding protein 3; IGFBP 3; IGFBP3

Expression system for standard: NS0; Immunogen sequence: A27-R291

There is no detectable cross-reactivity with other relevant proteins.

156 pg/ml - 10,000 pg/ml

IGFBP3

insulin-like growth factor binding protein 3

P17936  

Human IGFBP3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0386)

Insulin-like growth factor (IGF)-binding protein-3 (IGFBP-3) is a major determinant of circulating levels of the IGFs and is clinically useful for the evaluation of GH deficiency and for predicting the response to GH treatment. The circulating level of IGFBP-3 is inversely related to the risk of several common cancers, and that antiproliferative agents such as antiestrogens and retinoids act in part by up-regulating IGFBP-3 gene (IGFBP3) expression. Insulin-like growth factor-binding protein (IGFBP)-3, well characterized as the carrier of insulin-like growth factor (IGF), has been reported to have intrinsic bioactivity that is independent of IGF binding. IGFBP-3 has an IGF-independent, antiproliferative effect in undifferentiated and early differentiated but not in terminally differentiated chondrocytes. IGFBP-3 possesses both growth-inhibitory and potentiating effects on cells that are independent of IGF action and are mediated through specific. IGFBP-3 binding proteins/receptors locate at the cell membrane, cytosol, or nuclear compartments and in the extrIGFBP-3llular matrix. IGFBP3 is also located on chromosome 7. The standard product used in this kit is recombinant human IGFBP-3, consisting of 265 amino acids with the molecular mass of 29kDa. As a result of glycosylation, the molecular mass is 41KDa.

15-20分钟

Catalog number	Description	Quantity
EK0386-CAP	96-well plate precoated with anti- Human IGFBP3 ELISA Kit A antibody	1
EK0386-ST	Lyophilized recombinant Human IGFBP3 ELISA Kit A standard	10ng/tube×2
EK0386 -DA	Biotinylated anti- Human IGFBP3 ELISA Kit A antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	271	1607	5480	253	1639	5548
Standard deviation	16.53	69.1	350.72	19.73	95.06	437.8
CV(%)	6.1%	4.3%	6.4%	7.8%	5.8%	7.9%

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.002	0.058	0.112	0.237	0.485	0.923	1.701	2.554

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

human

酶联免疫双抗夹心法

cell culture supernatants, serum and plasma (heparin, EDTA).

3.5h