Mouse IGFBP3 ELISA Kit

96T|2480

BP 53; IBP 3; IBP3; IGF binding protein 3; IGFBP 3; IGFBP3

Expression system for standard: NS0; Immunogen sequence: G28Q-K291

There is no detectable cross-reactivity with other relevant proteins.

156pg/ml-10000pg/ml

IGFBP3

insulin-like growth factor binding protein 3

B3KPF0  

Mouse IGFBP3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0387)

Insulin-like growth factor (IGF)-binding protein-3 (IGFBP-3) is a major determinant of circulating levels of the IGFs and is clinically useful for the evaluation of GH deficiency and for predicting the response to GH treatment. The circulating level of IGFBP-3 is inversely related to the risk of several common cancers, and that antiproliferative agents such as antiestrogens and retinoids act in part by up-regulating IGFBP-3 gene (IGFBP3) expression. Insulin-like growth factor-binding protein (IGFBP)-3, well characterized as the carrier of insulin-like growth factor (IGF), has been reported to have intrinsic bioactivity that is independent of IGF binding. IGFBP-3 has an IGF-independent, antiproliferative effect in undifferentiated and early differentiated but not in terminally differentiated chondrocytes. IGFBP-3 possesses both growth-inhibitory and potentiating effects on cells that are independent of IGF action and are mediated through specific. IGFBP-3 binding proteins/receptors locate at the cell membrane, cytosol, or nuclear compartments and in the extrIGFBP-3llular matrix. IGFBP3 is also located on chromosome 7. The standard product used in this kit is recombinant mouse IGFBP-3, consisting of 270 amino acids with the molecular mass of 29.4kDa. As a result of glycosylation, the molecular mass is 40-50KDa.

Catalog number	Description	Quantity
EK0387-CAP	96-well plate precoated with anti- mouse IGFBP-3 antibody	1
EK0387-ST	Lyophilized recombinant mouse IGFBP-3 standard	10ng/tubex2
EK0387-DA	Biotinylated anti- mouse IGFBP-3 antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	269	1078	5499	266	1016	5861
Standard deviation	13.98	58.21	335.43	15.42	61.97	380.96
CV(%)	5.2%	5.4%	6.1%	5.8%	6.1%	6.5%

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.016	0.07	0.128	0.248	0.458	0.817	1.452	2.011

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants and Serum

3.5h