Human IGFBP3 EZ-Set™ ELISA Kit (DIY Antibody Pairs)

5 plates(kit)|3980

Acid stable subunit of the 140 K IGF complex|Binding protein 29|Binding protein 53|BP 53|BP53|Growth hormone dependent binding protein|IBP 3|IBP3|IGF binding protein 3|IGFBP 3|Insulin Like Growth Factor Binding Protein 3

Expression system for standard: NS0; Immunogen sequence: A28Q-291

There is no detectable cross-reactivity with other relevant proteins.

156 pg/ml - 10,000 pg/ml

IGFBP3

Insulin-like growth factor-binding protein 3;IBP-3;IGF-binding protein 3;IGFBP-3;IGFBP3;IBP3;

P17936  

Human IGFBP3 EZ-Set™ ELISA Kit (DIY Antibody Pairs) (Boster Biological Technology, Wuhan, China. Catalog#EZ0386)

Insulin-like growth factor(IGF)-binding protein-3(IGFBP-3) is a major determinant of circulating levels of the IGFs and is clinically useful for the evaluation of GH deficiency and for predicting the response to GH treatment. The circulating level of IGFBP-3 is inversely related to the risk of several common cancers, and that antiproliferative agents such as antiestrogens and retinoids act in part by up-regulating IGFBP-3 gene(IGFBP3) expression. Insulin-like growth factor-binding protein(IGFBP)-3, well characterized as the carrier of insulin-like growth factor(IGF), has been reported to have intrinsic bioactivity that is independent of IGF binding. IGFBP-3 has an IGF-independent, antiproliferative effect in undifferentiated and early differentiated but not in terminally differentiated chondrocytes. IGFBP-3 possesses both growth-inhibitory and potentiating effects on cells that are independent of IGF action and are mediated through specific. IGFBP-3 binding proteins/receptors locate at the cell membrane, cytosol, or nuclear compartments and in the extrIGFBP-3llular matrix. IGFBP3 is also located on chromosome 7. The standard product used in this kit is recombinant human IGFBP-3, consisting of 265 amino acids with the molecular mass of 29kDa. As a result of glycosylation, the molecular mass is 41KDa.

Catalog number	Description	Quantity	Storage of opened/reconstituted material
EZ0386-CA	Rabbit anti- human IGFBP3 polyclonal antibody (Capture Antibody)	500 µl, 0.3 mg/mL (recommended dilution 1:100)	Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
EZ0386-DA	Biotinylated goat anti- human IGFBP3 polyclonal antibody (Detection Antibody)	500 µl, 2 µg/mL (recommended dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	500 µl (recommended dilution 1:100)
EZ0386-ST	Lyophilized recombinant human IGFBP3 standard	10 ng/tube×3	Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.034	0.092	0.16	0.23	0.397	0.691	1.251	1.71

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate (Cat# AR1100)
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution:
Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
12.Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
*样品准备的相关试剂也不含在试剂盒中。

human

Insulin-like growth factor-binding protein 3

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)