产品名称
Human MMP9 ELISA Kit
指标别名
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; Gelatinase B; GELB; Matrix metalloproteinase 9; MMP 9; MMP9; MMP-9; MMP9 (N-terminal)
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名全称
matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
文献引用格式
Human MMP9 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0465)
背景资料
The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9(MMP9). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases. The matrix metalloproteinases(MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema. The standard product used in this kit is recombinant human MMP-9 with the molecular mass of 95KDa. The detected MMP-9 includes zymogen and active enzyme.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0465-CAP | 96-well plate precoated with anti- human MMP-9 antibody | 1 |
EK0465-ST | Lyophilized recombinant human MMP-9 standard | 10ng/tubex2 |
EK0465-DA | Biotinylated anti- human MMP-9 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 237 | 1530 | 5547 | 250 | 1429 | 5731 |
Standard deviation | 18.72 | 117.81 | 438.21 | 23.5 | 135.75 | 504.32 |
CV(%) | 7.9 | 7.7 | 7.9 | 9.4 | 9.5 | 8.8 |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.007 | 0.060 | 0.112 | 0.231 | 0.451 | 0.819 | 1.430 | 2.301 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin), Saliva and Urine