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Human MMP9 ELISA Kit

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EK0465

  • 96T ¥2480
  • 货期: 现货

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产品名称
Human MMP9 ELISA Kit
规格/价格
96T/2480
指标别名
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; Gelatinase B; GELB; Matrix metalloproteinase 9; MMP 9; MMP9; MMP-9; MMP9 (N-terminal)
免疫原
NSO, A20-D707
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名称
MMP9
基因名全称
matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
Uniprot ID
P14780  
文献引用格式
Human MMP9 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0465)
背景资料
The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9(MMP9). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases. The matrix metalloproteinases(MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema. The standard product used in this kit is recombinant human MMP-9 with the molecular mass of 95KDa. The detected MMP-9 includes zymogen and active enzyme.
显色时间
25-30分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0465-CAP96-well plate precoated with anti- human MMP-9 antibody1
EK0465-STLyophilized recombinant human MMP-9 standard10ng/tubex2
EK0465-DABiotinylated anti- human MMP-9 antibody100ul(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex(ABC)100ul(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)2371530554725014295731
Standard deviation18.72117.81438.2123.5135.75504.32
CV(%)7.97.77.99.49.58.8
典型数据示例
Concentration(pg/ml)015631262512502500500010000
O.D.0.0070.0600.1120.2310.4510.8191.4302.301
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin), Saliva and Urine
实验时长
3.5h

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