产品名称
Mouse MMP9 ELISA Kit
指标别名
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; Gelatinase B; GELB; Matrix metalloproteinase 9; MMP 9; MMP9; MMP-9; MMP9 (N-terminal)
免疫原
Expression system for standard: CHO; Immunogen sequence: A20-P730
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Matrix metalloproteinase-9
文献引用格式
Mouse MMP9 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0466)
背景资料
The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9 (MMP9). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases.1 The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0466-CAP | 96-well plate precoated with anti- mouse MMP-9 antibody | 1 |
| EK0466-ST | Lyophilized recombinant mouse MMP-9 standard | 10ng/tubex2 |
| EK0466-DA | Biotinylated anti- mouse MMP-9 antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 205 | 1787 | 4289 | 222 | 1732 | 4214 |
| Standard deviation | 15.37 | 141.17 | 214.45 | 18.64 | 143.75 | 214.91 |
| CV(%) | 7.5% | 7.9% | 5% | 8.4% | 8.3% | 5.1% |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.026 | 0.078 | 0.111 | 0.195 | 0.377 | 0.659 | 1.166 | 1.912 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin)