指标别名
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; Gelatinase B; GELB; Matrix metalloproteinase 9; MMP 9; MMP9; MMP-9; MMP9 (N-terminal)
免疫原
Expression system for standard: NS0; Immunogen sequence: L35-P708
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
matrix metallopeptidase 9
文献引用格式
Rat MMP9 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1463)
背景资料
Matrix metallopeptidase 9 (MMP-9) is also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases. The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema.
试剂盒内容
Catalog number | Description | Quantity |
EK1463-CAP | 96-well plate precoated with anti- rat MMP-9 antibody | 1 |
EK1463-ST | Lyophilized rat MMP-9 standard | 20ng/tube×2 |
EK1463-DA | Biotinylated anti- rat MMP-9 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 429 | 2362 | 8880 | 450 | 2199 | 8392 |
Standard deviation | 29.17 | 153.53 | 594.96 | 33.75 | 167.12 | 646.18 |
CV(%) | 6.8% | 6.5% | 6.7% | 7.5% | 7.6% | 7.7% |
典型数据示例
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.010 | 0.031 | 0.059 | 0.119 | 0.287 | 0.720 | 1.590 | 2.334 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin)