Human E-Selectin/CD62E/SELE ELISA Kit

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EK0501

96T ￥2280
货期：现货

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Human sE-Selectin ELISA Kit standard curve
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产品简介>

产品名称

Human E-Selectin/CD62E/SELE ELISA Kit

规格/价格

96T/2280

指标别名

CD62E; ELAM; ELAM 1; ELAM1; ESEL; E-selectin; E-selectin /CD62E; LECAM2; SELE; selectin E

免疫原

NSO, W22-P556

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

125 pg/ml - 8000 pg/ml

基因名称

SELE

基因名全称

selectin E

Uniprot ID

P16581

文献引用格式

Human E-Selectin/CD62E/SELE ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0501)

背景资料

E-selectin, also called endothelial-leukocyte adhesion molecule-1(ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells(HPCs) into bone marrow(BM). A structurally and functionally related group of genes, lymph node homing receptor(LHR), granule membrane protein 140(GMP-140), and endothelial leukocyte adhesion molecule 1(ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. The standard product used in this kit is recombinant human E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 535 amino acids with the molecular mass of 58.6KDa.

显色时间

25-30分钟

试剂盒内容

Catalog number	Description	Quantity
EK0501-CAP	96-well plate precoated with anti- human sE-Selectin antibody	1
EK0501-ST	Lyophilized recombinant human sE-Selectin standard	10ng/tubex2
EK0501-DA	Biotinylated anti- human sE-Selectin antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	152	1179	5147	138	1081	5271
Standard deviation	12	70.74	303.67	11.04	76.75	390.05
CV(%)	7.9	6	5.9	8	7.1	7.4

典型数据示例

Concentration(pg/ml)	0	125	250	500	1000	2000	4000	8000
O.D.	0.005	0.212	0.413	0.676	1.116	1.491	1.894	2.088

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Human E-Selectin/CD62E/SELE ELISA Kit

EK0501

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

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引用文献(0)>

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Human E-Selectin/CD62E/SELE ELISA Kit

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