产品名称
Rat E-selectin/CD62E/SELE Quick ELISA Kit
指标别名
CD62E; ELAM; ELAM 1; ELAM1; ESEL; E-selectin; E-selectin /CD62E; LECAM2; SELE; selectin E
免疫原
Expression system for standard: NS0; Immunogen sequence: W22-P494
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Rat E-selectin/CD62E/SELE Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0936)
背景资料
E-selectin, also called endothelial-leukocyte adhesion molecule-1(ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils.1 An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions.2 E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells(HPCs) into bone marrow(BM).3 A structurally and functionally related group of genes, lymph node homing receptor(LHR), granule membrane protein 140(GMP-140), and endothelial leukocyte adhesion molecule 1(ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1.4
试剂盒内容
Catalog number | Description | Quantity |
FEK0936-CAP | Anti-Pre-coated 96-well Strip Microplate | 1 |
FEK0936-ST | Rat E-selectin standard | 10ng/tube×2 |
FEK0936-A | Rat E-selectin Antibody A | 3ml |
FEK0936-B | Rat E-selectin Antibody B | 3ml |
AR1106-1 | Sample diluent buffer | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | Stop solution | 10ml |
PLA-SEA | Adhesive cover | 2 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 345 | 1737 | 4428 | 315 | 1621 | 4077 |
Standard deviation | 17.68 | 132.01 | 292.24 | 14.8 | 149.13 | 309.85 |
CV(%) | 4.4% | 7.6% | 6.6% | 4.7% | 9.2% | 7.6% |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.007 | 0.153 | 0.275 | 0.514 | 0.860 | 1.224 | 1.607 | 1.831 |
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, citrate)