产品名称
Rat E-selectin/CD62E/SELE ELISA Kit
指标别名
CD62E; ELAM; ELAM 1; ELAM1; ESEL; E-selectin; E-selectin /CD62E; LECAM2; SELE; selectin E
免疫原
Expression system for standard: NS0; Immunogen sequence: W22-P494
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Rat E-selectin/CD62E/SELE ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0936)
背景资料
E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils.1 An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions.2 E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM).3 A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1.4
试剂盒内容
| Catalog number | Description | Quantity |
| EK0936-CAP | 96-well plate precoated with anti- rat E-Selectin antibody | 1 |
| EK0936-ST | Lyophilized recombinant rat E-Selectinstandard | 10ng/tube×2 |
| EK0936-DA | Biotinylated anti- rat E-Selectin antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 345 | 1737 | 4428 | 315 | 1621 | 4077 |
| Standard deviation | 15.18 | 132.01 | 292.24 | 14.8 | 149.13 | 309.85 |
| CV(%) | 4.4% | 7.6% | 6.6% | 4.7% | 9.2% | 7.6% |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.008 | 0.086 | 0.174 | 0.331 | 0.637 | 1.052 | 1.614 | 2.2 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, citrate)