产品名称
Human CD56/NCAM1 ELISA Kit
指标别名
CD56; MSK39; N CAM 1; NCAM; NCAM 1; NCAM1; NCAM1/CD56
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名全称
neural cell adhesion molecule 1
文献引用格式
Human CD56/NCAM1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0706)
背景资料
Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin(Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23. This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell–cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0706-CAP | 96-well plate precoated with anti- human CD56 antibody | 1 |
| EK0706-ST | Lyophilized recombinant human CD56 standard | 10ng/tube×2 |
| EK0706-DA | Biotinylated anti- human CD56 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 319 | 1085 | 5570 | 313 | 1102 | 5558 |
| Standard deviation | 16.26 | 44.48 | 239.51 | 17.21 | 50.69 | 316.8 |
| CV(%) | 5.1 | 4.1 | 4.3 | 5.5 | 4.6 | 5.7 |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.029 | 0.095 | 0.155 | 0.241 | 0.432 | 0.76 | 1.3 | 1.838 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)