产品名称
Mouse CD56/NCAM1 ELISA Kit
指标别名
CD56; MSK39; N CAM 1; NCAM; NCAM 1; NCAM1; NCAM1/CD56
免疫原
Expression system for standard: NS0; Immunogen sequence: L20-T711
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Neural cell adhesion molecule 1
文献引用格式
Mouse CD56/NCAM1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1223)
背景资料
Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin (Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23.This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell–cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1223-CAP | 96-well plate precoated with anti- mouse CD56 antibody | 1 |
| EK1223-ST | Lyophilized recombinant mouse CD56 standard | 40ng/tube×2 |
| EK1223-DA | Biotinylated anti- mouse CD56 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 1344 | 4632 | 15378 | 1401 | 4645 | 16195 |
| Standard deviation | 90.04 | 342.76 | 953.43 | 120.48 | 422.69 | 1214.62 |
| CV(%) | 6.7% | 7.4% | 6.2% | 8.6% | 9.1% | 7.5% |
典型数据示例
| Concentration (ng/ml) | 0 | 0.625 | 1.25 | 2.5 | 5 | 10 | 20 | 40 |
| O.D. | 0.038 | 0.143 | 0.268 | 0.411 | 0.772 | 1.3 | 1.634 | 1.944 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)