产品名称
Rat CD56/NCAM1 ELISA Kit
指标别名
CD56; MSK39; N CAM 1; NCAM; NCAM 1; NCAM1; NCAM1/CD56
免疫原
Expression system for standard: NS0; Immunogen sequence: L20-T712
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
neural cell adhesion molecule 1
文献引用格式
Rat CD56/NCAM1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1562)
背景资料
Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell–cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory. This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. And the encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance.
试剂盒内容
| Catalog number | Description | Quantity |
| EK1562-CAP | 96-well plate precoated with anti- rat CD56 antibody | 1 |
| EK1562-ST | Lyophilized rat CD56 standard | 40ng/tube×2 |
| EK1562-DA | Biotinylated anti- rat CD56 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 1208 | 4356 | 19046 | 1152 | 4200 | 19885 |
| Standard deviation | 57.98 | 204.73 | 1047.53 | 72.57 | 201.6 | 1411.83 |
| CV(%) | 4.8% | 4.7% | 5.5% | 6.3% | 4.8% | 7.1% |
典型数据示例
| Concentration (ng/ml) | 0 | 0.625 | 1.25 | 2.5 | 5 | 10 | 20 | 40 |
| O.D. | 0.007 | 0.082 | 0.164 | 0.264 | 0.521 | 0.984 | 1.692 | 2.055 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)