Anti-SHP2/PTPN11 Antibody (Clone#2E6)

Mouse monoclonal antibody

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筛选器： All WB IHC ICC/IF FCM

M00150-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of SHP2/PTPN11 using anti-SHP2/PTPN11 antibody (M00150-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-SHP2/PTPN11 antigen affinity purified monoclonal antibody (M00150-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SHP2/PTPN11 at approximately 68 kDa. The expected band size for SHP2/PTPN11 is at 68 kDa.
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IHC analysis of SHP2/PTPN11 using anti-SHP2/PTPN11 antibody (M00150-2).
SHP2/PTPN11 was detected in a paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-SHP2/PTPN11 Antibody (M00150-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of SHP2/PTPN11 using anti-SHP2/PTPN11 antibody (M00150-2).
SHP2/PTPN11 was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-SHP2/PTPN11 Antibody (M00150-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of SHP2/PTPN11 using anti-SHP2/PTPN11 antibody (M00150-2).
SHP2/PTPN11 was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-SHP2/PTPN11 Antibody (M00150-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of SHP2/PTPN11 using anti-SHP2/PTPN11 antibody (M00150-2).
SHP2/PTPN11 was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-SHP2/PTPN11 Antibody (M00150-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(7)
ICC/IF analysis of SHP2/PTPN11 using anti- SHP2/PTPN11 antibody (M00150-2). SHP2/PTPN11 was detected in immunocytochemical section of U251 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti- SHP2/PTPN11 Antibody (M00150-2) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Flow cytometry analysis of A549 cell (1:100) Fluoro 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG Fluoro 488. Unlabelled sample (Red line).
all(7)

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产品简介>

产品名称

Anti-SHP2/PTPN11 Antibody (Clone#2E6)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

BPTP3; CFC; NS1; PTP 1D; PTP 2C; PTP2C; PTPN11; SH PTP2; SH PTP3; SHP 2; SHP2; SHPTP2

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, FCM

基因名称

PTPN11

克隆号

2E6

宿主

Mouse

抗体亚型

IgG2b

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human SHP2, identical to the related mouse and rat sequences.

计算分子量

68 kDa

实际分子量

68 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

200ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

PTPN11 (Tyrosine-protein phosphatase non-receptor type 11), also known as protein-tyrosine phosphatase 1D (PTP-1D), protein-tyrosine phosphatase 2C (PTP-2C), TYROSINE PHOSPHATASE SHP2 (SHP2), BPTP3, SH-PTP2, SHP-2, SH-PTP3, is an enzyme that in humans is encoded by the PTPN11 gene. PTPN11 is a member of the protein tyrosine phosphatase (PTP) family. The open reading frame consists of 1,779 nucleotides potentially encoding a protein of 593 amino acids with a predicted molecular mass of 68 kD. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia.

Uniprot ID

Q06124

RRID

AB_3081747

文献引用格式

SHP2/PTPN11 Antibody (Clone#2E6) (Boster Biological Technology, Wuhan, China. Catalog#M00150-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

实验方案>

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