Anti-CDK2 Antibody (Clone#OTI1C4)

Mouse monoclonal antibody

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M00166-5

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and CDK2-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-CDK2 monoclonal antibody M00166-5, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody ([M01692-2]) as a loading control.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CDK2 monoclonal antibody.
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HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDK2 antibody, and then analyzed by flow cytometry.
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Flow cytometric Analysis of Hela cells, using anti-CDK2 antibody, (Red), compared to PBS (green) and a nonspecific negative control antibody, (Blue) (1:100).
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Immunofluorescent staining of HeLa cells using anti-CDK2 mouse monoclonal antibody.
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Anti-CDK2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CDK2 .
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDK2.
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Western blot analysis of extracts (35ug) from 14 cell lines by using anti-CDK2 monoclonal antibody (1:500).
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产品简介>

产品名称

Anti-CDK2 Antibody (Clone#OTI1C4)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CDK2; Cell division protein kinase 2; cyclin dependent kinase 2; p33 protein kinase; p33(CDK2)

产品类型

Monoclonal

检验物种

human, mouse, rat, monkey

应用范围

WB, ICC/IF, FCM

基因名称

CDK2

克隆号

OTI1C4

宿主

Mouse

抗体亚型

IgG2a

免疫原

Full length human recombinant protein of human CDK2 (NP_001789) produced in HEK293T cell.

计算分子量

34 kDa

实际分子量

33.7 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

CDK2, Cyclin-Dependent Kinase2, is also known as P33. The CDK2 protein was highly homologous to p34(CDC2) kinase and more significantly homologous to Xenopus Eg1 kinase, suggesting that CDK2 is the human homolog of Eg1. The CDK2 gene is mapped to 12q13, the same region to which the CDK4 gene maps. Human cyclin A binds independently to 2 kinases, p34(cdc2) or p33. In adenovirus-transformed cells, the viral E1A oncoprotein seems to associate with p33/cyclin A but not with p34(cdc2)/cyclin A. The gene for p33 shares 65% sequence identity with p34(cdc2). P33(cdk2) plays a unique role in cell cycle regulation of vertebrate cells.

Uniprot ID

P24941

文献引用格式

CDK2 Antibody (Clone#OTI1C4) (Boster Biological Technology, Wuhan, China. Catalog#M00166-5)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

cyclin-dependent kinase 2

蛋白名全称

Cyclin-dependent kinase 2

实验方案>

实验步骤

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