Western blot analysis of CDK2 using anti-CDK2 antibody (M00166-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Jurkat whole cell lysates,
Lane 2: HepG2 whole cell lysates,
Lane 3: K562 whole cell lysates,
Lane 4: rat heart tissue lysates,
Lane 5: L6 whole cell lysates,
Lane 6: mouse heart tissue lysates,
Lane 7: C2C12 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-CDK2 antigen affinity purified monoclonal antibody (M00166-3) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CDK2 at approximately 30-34 kDa. The expected band size for CDK2 is at 34 kDa.
IHC analysis of CDK2 using anti-CDK2 antibody (M00166-3).
CDK2 was detected in a paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CDK2 Antibody (M00166-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CDK2 using anti-CDK2 antibody (M00166-3).
CDK2 was detected in a paraffin-embedded section of human Laryngeal squamous cell carcinomas tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CDK2 Antibody (M00166-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CDK2 using anti-CDK2 antibody (M00166-3).
CDK2 was detected in a paraffin-embedded section of human Serous adenocarcinoma of ovary tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CDK2 Antibody (M00166-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CDK2 using anti-CDK2 antibody (M00166-3).
CDK2 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CDK2 Antibody (M00166-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of ANA-1 cells using anti-CDK2 antibody (M00166-3).
Overlay histogram showing ANA-1 cells stained with M00166-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CDK2 Antibody (M00166-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of CDK2 using anti-CDK2 antibody (M00166-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Jurkat whole cell lysates,
Lane 2: HepG2 whole cell lysates,
Lane 3: K562 whole cell lysates,
Lane 4: rat heart tissue lysates,
Lane 5: L6 whole cell lysates,
Lane 6: mouse heart tissue lysates,
Lane 7: C2C12 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-CDK2 antigen affinity purified monoclonal antibody (M00166-3) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CDK2 at approximately 30-34 kDa. The expected band size for CDK2 is at 34 kDa.
IHC analysis of CDK2 using anti-CDK2 antibody (M00166-3).
CDK2 was detected in a paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CDK2 Antibody (M00166-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CDK2 using anti-CDK2 antibody (M00166-3).
CDK2 was detected in a paraffin-embedded section of human Laryngeal squamous cell carcinomas tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CDK2 Antibody (M00166-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CDK2 using anti-CDK2 antibody (M00166-3).
CDK2 was detected in a paraffin-embedded section of human Serous adenocarcinoma of ovary tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CDK2 Antibody (M00166-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CDK2 using anti-CDK2 antibody (M00166-3).
CDK2 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CDK2 Antibody (M00166-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of ANA-1 cells using anti-CDK2 antibody (M00166-3).
Overlay histogram showing ANA-1 cells stained with M00166-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CDK2 Antibody (M00166-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Western blot analysis of CDK2 using anti-CDK2 antibody (M00166-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Jurkat whole cell lysates,
Lane 2: HepG2 whole cell lysates,
Lane 3: K562 whole cell lysates,
Lane 4: rat heart tissue lysates,
Lane 5: L6 whole cell lysates,
Lane 6: mouse heart tissue lysates,
Lane 7: C2C12 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-CDK2 antigen affinity purified monoclonal antibody (M00166-3) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CDK2 at approximately 30-34 kDa. The expected band size for CDK2 is at 34 kDa.
IHC analysis of CDK2 using anti-CDK2 antibody (M00166-3).
CDK2 was detected in a paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CDK2 Antibody (M00166-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CDK2 using anti-CDK2 antibody (M00166-3).
CDK2 was detected in a paraffin-embedded section of human Laryngeal squamous cell carcinomas tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CDK2 Antibody (M00166-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CDK2 using anti-CDK2 antibody (M00166-3).
CDK2 was detected in a paraffin-embedded section of human Serous adenocarcinoma of ovary tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CDK2 Antibody (M00166-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CDK2 using anti-CDK2 antibody (M00166-3).
CDK2 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CDK2 Antibody (M00166-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of ANA-1 cells using anti-CDK2 antibody (M00166-3).
Overlay histogram showing ANA-1 cells stained with M00166-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CDK2 Antibody (M00166-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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