Anti-CDK2 Antibody (Clone#6D5B5)

Mouse monoclonal antibody

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筛选器： All WB IHC

M00166-4

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CDK2 using anti-CDK2 antibody (M00166-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Jurkat whole cell lysates,
Lane 2: HepG2 whole cell lysates,
Lane 3: U2OS whole cell lysates,
Lane 4: rat heart tissue lysates,
Lane 5: L6 whole cell lysates,
Lane 6: mouse heart tissue lysates,
Lane 7: C2C12 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-CDK2 antigen affinity purified monoclonal antibody (M00166-4) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CDK2 at approximately 30-34 kDa. The expected band size for CDK2 is at 34 kDa.
all(7)
IHC analysis of CDK2 using anti-CDK2 antibody (M00166-4).
CDK2 was detected in a paraffin-embedded section of human Laryngeal squamous cell carcin tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CDK2 Antibody (M00166-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of CDK2 using anti-CDK2 antibody (M00166-4).
CDK2 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CDK2 Antibody (M00166-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of CDK2 using anti-CDK2 antibody (M00166-4).
CDK2 was detected in a paraffin-embedded section of human renal clear cell carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CDK2 Antibody (M00166-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of CDK2 using anti-CDK2 antibody (M00166-4).
CDK2 was detected in a paraffin-embedded section of human Serous adenocarcinoma of ovary tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CDK2 Antibody (M00166-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of CDK2 using anti-CDK2 antibody (M00166-4).
CDK2 was detected in a paraffin-embedded section of mouse colon tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CDK2 Antibody (M00166-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of CDK2 using anti-CDK2 antibody (M00166-4).
CDK2 was detected in a paraffin-embedded section of mouse colon tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CDK2 Antibody (M00166-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(7)

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产品简介>

产品名称

Anti-CDK2 Antibody (Clone#6D5B5)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CDK2; Cell division protein kinase 2; cyclin dependent kinase 2; p33 protein kinase; p33(CDK2)

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

CDK2

克隆号

6D5B5

宿主

Mouse

抗体亚型

IgG2b

免疫原

E.coli-derived human Cdk2 recombinant protein (Position: E81-L298). Human Cdk2 shares 98.6% amino acid (aa) sequence identity with rat Cdk2.

计算分子量

34 kDa

实际分子量

30-34 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

CDK2, Cyclin-Dependent Kinase2, is also known as P33. The CDK2 protein was highly homologous to p34(CDC2) kinase and more significantly homologous to Xenopus Eg1 kinase, suggesting that CDK2 is the human homolog of Eg1. The CDK2 gene is mapped to 12q13, the same region to which the CDK4 gene maps. Human cyclin A binds independently to 2 kinases, p34(cdc2) or p33. In adenovirus-transformed cells, the viral E1A oncoprotein seems to associate with p33/cyclin A but not with p34(cdc2)/cyclin A. The gene for p33 shares 65% sequence identity with p34(cdc2). P33(cdk2) plays a unique role in cell cycle regulation of vertebrate cells.

Uniprot ID

P24941

文献引用格式

CDK2 Antibody (Clone#6D5B5) (Boster Biological Technology, Wuhan, China. Catalog#M00166-4)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

cyclin-dependent kinase 2

蛋白名全称

Cyclin-dependent kinase 2

实验方案>

实验步骤

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