Anti-CDK2 (Phospho-y15) Antibody (Clone#GBB-3)

Rabbit Monoclonal antibody

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筛选器： All WB IHC IP

BM4590

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-P-CDK2 antibody (BM4590). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: mouse Neuro-2a tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-P-CDK2 antigen affinity purified monoclonal antibody (BM4590) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for P-CDK2 at approximately 34 kDa. The expected band size for P-CDK2 is at 34 kDa.
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Immunohistochemical analysis of paraffin-embedded human bladder cancer, using Phospho-CDK2 (Y15) Antibody.
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产品简介>

产品名称

Anti-CDK2 (Phospho-y15) Antibody (Clone#GBB-3)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

CDK2; Cell division protein kinase 2; cyclin dependent kinase 2; p33 protein kinase; p33(CDK2)

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IP

基因名称

CDK2

克隆号

GBB-3

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human CDK2 CDK2 is an important component of the cell cycle machinery. Like p34cdc2, kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a CDK inhibitor. This protein can be regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B) .

计算分子量

34 kDa

实际分子量

30-34 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CDK2, Cyclin-Dependent Kinase2, is also known as P33. The CDK2 protein was highly homologous to p34(CDC2) kinase and more significantly homologous to Xenopus Eg1 kinase, suggesting that CDK2 is the human homolog of Eg1. The CDK2 gene is mapped to 12q13, the same region to which the CDK4 gene maps. Human cyclin A binds independently to 2 kinases, p34(cdc2) or p33. In adenovirus-transformed cells, the viral E1A oncoprotein seems to associate with p33/cyclin A but not with p34(cdc2)/cyclin A. The gene for p33 shares 65% sequence identity with p34(cdc2). P33(cdk2) plays a unique role in cell cycle regulation of vertebrate cells.

Uniprot ID

P24941

文献引用格式

CDK2 (Phospho-y15) Antibody (Clone#GBB-3) (Boster Biological Technology, Wuhan, China. Catalog#BM4590)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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