Anti-TIMP2 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

PB0875

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of TIMP2 using anti-TIMP2 antibody (PB0875). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat spleen tissue lysates,
Lane 2: Rat testis tissue lysates,
Lane 3: Mouse brain tissue lysates,
Lane 4: Mouse thymus tissue lysates,
Lane 5: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TIMP2 antigen affinity purified polyclonal antibody (PB0875) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TIMP2 at approximately 22 kDa. The expected band size for TIMP2 is at 24 kDa.
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产品简介>

产品名称

Anti-TIMP2 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

CSC 21K; Metalloproteinase inhibitor 2; TIMP 2; TIMP2; TIMP-2

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

TIMP2

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human TIMP2 recombinant protein (Position: C27-P220). Human TIMP2 shares 98.5% amino acid (aa) sequence identity with both mouse and rat TIMP2.

计算分子量

24 kDa

实际分子量

22 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

TIMP metallopeptidase inhibitor 2, a tissue inhibitor of metalloproteinases, also known as TIMP2, is a human gene, thought to be a metastasis suppressor. This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix.

Uniprot ID

P16035

文献引用格式

TIMP2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0875)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

TIMP metallopeptidase inhibitor 2

蛋白名全称

Metalloproteinase inhibitor 2

实验方案>

实验步骤

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