Western blot analysis of GRK2 using anti-GRK2 antibody (A32388-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Human THP-1 whole cell lysates,
Lane 2: Monkey COS-7 whole cell lysates,
Lane 3: Human Raji whole cell lysates,
Lane 4: Rat lung tissue lysates,
Lane 5: Mouse NIH/3T3 whole cell lysates,
Lane 6: Mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GRK2 antigen affinity purified polyclonal antibody (A32388-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GRK2 at approximately 80 kDa. The expected band size for GRK2 is at 80 kDa.

IF analysis of GRK2 using anti-GRK2 antibody (A32388-1).
GRK2 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-GRK2 Antibody (A32388-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

Flow Cytometry analysis of Hepa1-6 cells using anti-GRK2 antibody (A32388-1).
Overlay histogram showing Hepa1-6 cells stained with A32388-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRK2 Antibody (A32388-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Flow Cytometry analysis of RH-35 cells using anti-GRK2 antibody (A32388-1).
Overlay histogram showing RH-35 cells stained with A32388-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRK2 Antibody (A32388-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Flow Cytometry analysis of U937 cells using anti-GRK2 antibody (A32388-1).
Overlay histogram showing U937 cells stained with A32388-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRK2 Antibody (A32388-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Western blot analysis of GRK2 using anti-GRK2 antibody (A01473-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat stomach tissue lysates,
Lane 3: mouse lung tissue lysates,
Lane 4: mouse liver tissue lysates,
Lane 5: mouse pancreas tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GRK2 antigen affinity purified polyclonal antibody (A01473-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GRK2 at approximately 80 kDa. The expected band size for GRK2 is at 80 kDa.

IF analysis of GRK2 using anti-GRK2 antibody (A01473-1).
GRK2 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-GRK2 Antibody (A01473-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

Flow Cytometry analysis of U87 cells using anti-GRK2 antibody (A01473-1).
Overlay histogram showing U87 cells stained with A01473-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GRK2 Antibody (A01473-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Western blot analysis of GRK2 expression in THP-1 cell lysate.

Western blot analysis of ATG14 using anti-ATG14 antibody (PB9481). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Brain tissue lysates,
Lane 2: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATG14 antigen affinity purified polyclonal antibody (PB9481) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATG14 at approximately 59 kDa. The expected band size for ATG14 is at 55 kDa.

IHC analysis of ATG14 using anti-ATG14 antibody (PB9481).
ATG14 was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ATG14 Antibody (PB9481) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of ATG14 using anti-ATG14 antibody (PB9481).
ATG14 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ATG14 Antibody (PB9481) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

IF analysis of ATG14 using anti-ATG14 antibody (PB9481).
ATG14 was detected in an immunocytochemical section of U2OS cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

IF analysis of ATG14 using anti-ATG14 antibody (PB9481).
ATG14 was detected in a paraffin-embedded section of human lung cancer tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

IF analysis of ATG14 using anti-ATG14 antibody (PB9481).
ATG14 was detected in a paraffin-embedded section of human colon cancer tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

IF analysis of ATG14 using anti-ATG14 antibody (PB9481).
ATG14 was detected in a paraffin-embedded section of rat spleen tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

Flow Cytometry analysis of SiHa cells using anti-ATG14 antibody (PB9481).
Overlay histogram showing SiHa cells stained with PB9481 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG14 Antibody (PB9481) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Flow Cytometry analysis of A431 cells using anti-ATG14 antibody (PB9481).
Overlay histogram showing A431 cells stained with PB9481 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG14 Antibody (PB9481) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Flow Cytometry analysis of PC-3 cells using anti-ATG14 antibody (PB9481).
Overlay histogram showing PC-3 cells stained with PB9481 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG14 Antibody (PB9481) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Western blot analysis of GRK3 using anti-GRK3 antibody (A32254-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HL-60 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GRK3 antigen affinity purified polyclonal antibody (A32254-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GRK3 at approximately 80 kDa. The expected band size for GRK3 is at 80 kDa.

Western blot analysis of GRK3 using anti-GRK3 antibody (PB0744). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: JURKAT whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GRK3 antigen affinity purified polyclonal antibody (PB0744) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GRK3 at approximately 80 kDa. The expected band size for GRK3 is at 80 kDa.

IF analysis of anti-ADRBK2 antibody (PB0744).ADRBK2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ADRBK2 antibody (PB0744). overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Flow Cytometry analysis of A431 cells using anti-GRK3 antibody (PB0744).
Overlay histogram showing A431 cells stained with PB0744 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRK3 Antibody (PB0744) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Western blot analysis of GRK3 expression in Ramos cell lysate.