Anti-14-3-3 GAMMA/YWHAG-Specific Antibody

筛选器： All WB IHC FCM ELISA

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  Figure 1. Western blot analysis of 14-3-3 GAMMA/YWHAG-Specific using anti-14-3-3 GAMMA/YWHAG-Specific antibody (A04148-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Jurkat whole cell lysates,
  Lane 2: human placenta tissue lysates,
  Lane 3: rat brain tissue lysates,
  Lane 4: rat skeletal muscle tissue lysates,
  Lane 5: mouse brain tissue lysates,
  Lane 6: mouse skeletal muscle tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-14-3-3 GAMMA/YWHAG-Specific antigen affinity purified polyclonal antibody (A04148-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 14-3-3 GAMMA/YWHAG-Specific at approximately 28 kDa. The expected band size for 14-3-3 GAMMA/YWHAG-Specific is at 28 kDa.

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  Figure 2. IHC analysis of 14-3-3 GAMMA/YWHAG-Specific using anti-14-3-3 GAMMA/YWHAG-Specific antibody (A04148-2).14-3-3 GAMMA/YWHAG-Specific was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 GAMMA/YWHAG-Specific Antibody (A04148-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 3. Flow Cytometry analysis of U87 cells using anti-14-3-3GAMMA/YWHAG-Specific antibody (A04148-2).
  Overlay histogram showing U87 cells stained with A04148-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-14-3-3 GAMMA/YWHAG-Specific Antibody (A04148-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Anti-14-3-3 Epsilon/YWHAE Antibody

筛选器： All WB IHC FCM ICC/IF ELISA

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  Figure 1. Western blot analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (A01687-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human hela whole cell lysates,
  Lane 2: human Jurkat whole cell lysates,
  Lane 3: human hepg2 whole cell lysates,
  Lane 4: human SH-SY5Y whole cell lysates,
  Lane 5: human HEK293 whole cell lysates,
  Lane 6: human SW620 whole cell lysates,
  Lane 7: human A549 whole cell lysates,
  Lane 8: human Raji whole cell lysates,
  Lane 9: Rat brain tissue lysates,
  Lane 10: Mouse brain tissue lysates,
  Lane 11: Mouse NIH/3T3 whole cell lysates,
  Lane 12: Mouse RAW264.7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-14-3-3 Epsilon/YWHAE antigen affinity purified polyclonal antibody (A01687-4) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 14-3-3 Epsilon/YWHAE at approximately 29 kDa. The expected band size for 14-3-3 Epsilon/YWHAE is at 29 kDa.

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  Figure 2. IHC analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (A01687-4).
  14-3-3 Epsilon/YWHAE was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 Epsilon/YWHAE Antibody (A01687-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 3. Flow Cytometry analysis of A549 cells using anti-14-3-3 Epsilon/YWHAE antibody (A01687-4).
  Overlay histogram showing A549 cells stained with A01687-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-14-3-3 Epsilon/YWHAE Antibody (A01687-4) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Figure 4. IF analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (A01687-4).
  14-3-3 Epsilon/YWHAE was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-14-3-3 Epsilon/YWHAE Antibody (A01687-4) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

Anti-14-3-3 Epsilon/YWHAE Antibody

筛选器： All WB IHC ICC/IF ELISA

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  Figure 1. Western blot analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (A01687-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Hela whole cell lysates,
  Lane 2: Jurkat whole cell lysates,
  Lane 3: HepG2 whole cell lysates,
  Lane 4: 293T whole cell lysates,
  Lane 5: rat brain tissue lysates,
  Lane 6: rat PC-12 whole cell lysates,
  Lane 7: mouse brain tissue lysates,
  Lane 8: mouse NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-14-3-3 Epsilon/YWHAE antigen affinity purified polyclonal antibody (A01687-5) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 14-3-3 Epsilon/YWHAE at approximately 29 kDa. The expected band size for 14-3-3 Epsilon/YWHAE is at 29 kDa.

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  Figure 2. IHC analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (A01687-5).
  14-3-3 Epsilon/YWHAE was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 Epsilon/YWHAE Antibody (A01687-5) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 3. IHC analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (A01687-5).
  14-3-3 Epsilon/YWHAE was detected in a paraffin-embedded section of human prostate cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 Epsilon/YWHAE Antibody (A01687-5) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 4. IHC analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (A01687-5).
  14-3-3 Epsilon/YWHAE was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 Epsilon/YWHAE Antibody (A01687-5) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 5. IHC analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (A01687-5).
  14-3-3 Epsilon/YWHAE was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 Epsilon/YWHAE Antibody (A01687-5) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 6. IF analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (A01687-5).
  14-3-3 Epsilon/YWHAE was detected in an immunocytochemical section of A549 cells. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

Anti-14-3-3 GAMMA/YWHAG-Specific Antibody

筛选器： All WB FCM ELISA

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  Figure 1. Western blot analysis of 14-3-3 GAMMA/YWHAG-Specific using anti-14-3-3 GAMMA/YWHAG-Specific antibody (A04148-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat brain tissue lysates,
  Lane 2: Mouse brain tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-14-3-3 GAMMA/YWHAG-Specific antigen affinity purified polyclonal antibody (A04148-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 14-3-3 GAMMA/YWHAG-Specific at approximately 28 kDa. The expected band size for 14-3-3 GAMMA/YWHAG-Specific is at 28 kDa.

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  Figure 2. Flow Cytometry analysis of U87 cells using anti-14-3-3 GAMMA/YWHAG-Specific antibody (A04148-1).
  Overlay histogram showing U87 cells stained with A04148-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-14-3-3 GAMMA/YWHAG-Specific Antibody (A04148-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Anti-14-3-3 Sigma/SFN Antibody

筛选器： All WB IHC ICC/IF FCM ELISA

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  Figure 1. Western blot analysis of anti-14-3-3 Sigma/SFN antibody (A01127). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human A431 whole cell lysates,
  Lane 2: human Hela whole cell lysates,
  Lane 3: human MCF-7 whole cell lysates,
  Lane 4: human Hacat whole cell lysates,
  Lane 1: rat skin tissue lysates,
  Lane 2: mouse Hepa1-6 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-14-3-3 Sigma/SFN antigen affinity purified polyclonal antibody (A01127) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 14-3-3 Sigma/SFN at approximately 28 kDa. The expected band size for 14-3-3 Sigma/SFN is at 28 kDa.

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  Figure 2. IHC analysis of 14-3-3 Sigma/SFN using anti-14-3-3 Sigma/SFN antibody (A01127).
  14-3-3 Sigma/SFN was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 Sigma/SFN Antibody (A01127) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 3. IF analysis of 14-3-3 sigma using anti- 14-3-3 sigma antibody (A01127). 14-3-3 sigma was detected in paraffin-embedded section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti- cortactin Antibody (A01127) . DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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  Figure 4. IF analysis of 14-3-3 sigma using anti- 14-3-3 sigma antibody (A01127). 14-3-3 sigma was detected in paraffin-embedded section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti- cortactin Antibody (A01127) . DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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  Figure 5. Flow Cytometry analysis of U2OS cells using anti-14-3-3 Sigma/SFN antibody (A01127).
  Overlay histogram showing U2OS cells stained with A01127 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-14-3-3 Sigma/SFN Antibody (A01127) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Anti-14-3-3 Sigma/SFN Antibody

筛选器： All WB IHC ICC/IF FCM

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  Figure 1. Western blot analysis of 14-3-3 Sigma/SFN using anti-14-3-3 Sigma/SFN antibody (BA3752). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: HELA whole cell lysates,
  Lane 2: PC-3 whole cell lysates,
  Lane 3: rat brain tissue lysates,
  Lane 4: mouse brain tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-14-3-3 Sigma/SFN antigen affinity purified polyclonal antibody (BA3752) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 14-3-3 Sigma/SFN at approximately 28 kDa. The expected band size for 14-3-3 Sigma/SFN is at 28 kDa.

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  Figure 2. IHC analysis of 14-3-3 Sigma/SFN using anti-14-3-3 Sigma/SFN antibody (BA3752).
  14-3-3 Sigma/SFN was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 Sigma/SFN Antibody (BA3752) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 3. IHC analysis of 14-3-3 Sigma/SFN using anti-14-3-3 Sigma/SFN antibody (BA3752).
  14-3-3 Sigma/SFN was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 Sigma/SFN Antibody (BA3752) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 4. IHC analysis of 14-3-3 Sigma/SFN using anti-14-3-3 Sigma/SFN antibody (BA3752).
  14-3-3 Sigma/SFN was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 Sigma/SFN Antibody (BA3752) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 5. IF analysis of 14-3-3 Sigma/SFN using anti-14-3-3 Sigma/SFN antibody (BA3752).
  14-3-3 Sigma/SFN was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-14-3-3 Sigma/SFN Antibody (BA3752) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Figure 6. IF analysis of 14-3-3 Sigma/SFN using anti-14-3-3 Sigma/SFN antibody (BA3752).
  14-3-3 Sigma/SFN was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-14-3-3 Sigma/SFN Antibody (BA3752) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Figure 7. Flow Cytometry analysis of A431 cells using anti-14-3-3 Sigma/SFN antibody (BA3752).
  Overlay histogram showing A431 cells stained with BA3752 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-14-3-3 Sigma/SFN Antibody (BA3752) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Anti-14-3-3 Theta/YWHAQ Antibody

筛选器： All WB IHC ICC/IF FCM

Anti-15 Lipoxygenase 2/ALOX15B Antibody

筛选器： All WB ELISA

Anti-2B4/CD244 Antibody

筛选器： All WB ELISA

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  Figure 1. Western blot analysis of 2B4/CD244 using anti-2B4/CD244 antibody (A02527-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat spleen tissue lysates,
  Lane 2: rat spleen tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-2B4/CD244 antigen affinity purified polyclonal antibody (A02527-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 2B4/CD244 at approximately 42 kDa. The expected band size for 2B4/CD244 is at 42 kDa.

Anti-2B4/CD244 Antibody

筛选器： All WB ELISA

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  Figure 1. Western blot analysis of 2B4/CD244 using anti-2B4/CD244 antibody (A02527-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: mouse spleen tissue lysates,
  Lane 2: mouse thymus tissue lysates,
  Lane 3: mouse RAW264.7 whole cell lysates,
  Lane 3: mouse SP20 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-2B4/CD244 antigen affinity purified polyclonal antibody (A02527-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 2B4/CD244 at approximately 42 kDa. The expected band size for 2B4/CD244 is at 42 kDa.

Anti-4EBP1/EIF4EBP1 Antibody

筛选器： All WB IHC FCM

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  Figure 1. Western blot analysis of 4EBP1/EIF4EBP1 using anti-4EBP1/EIF4EBP1 antibody (A00968-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: HEL whole cell lysates,
  Lane 2: PC-3 whole cell lysates,
  Lane 3: HEK293 whole cell lysates,
  Lane 4: rat stomach tissue lysates,
  Lane 5: rat pancreas tissue lysates,
  Lane 6: mouse pancreas tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-4EBP1/EIF4EBP1 antigen affinity purified polyclonal antibody (A00968-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 4EBP1/EIF4EBP1 at approximately 17 kDa. The expected band size for 4EBP1/EIF4EBP1 is at 13 kDa.

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  Figure 2. IHC analysis of 4EBP1/EIF4EBP1 using anti-4EBP1/EIF4EBP1 antibody (A00968-1).
  4EBP1/EIF4EBP1 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-4EBP1/EIF4EBP1 Antibody (A00968-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 3. Flow Cytometry analysis of A431 cells using anti-4EBP1/EIF4EBP1 antibody (A00968-1).
  Overlay histogram showing A431 cells stained with A00968-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-4EBP1/EIF4EBP1 Antibody (A00968-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Anti-5 Lipoxygenase/ALOX5 Antibody

筛选器： All IHC WB

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  Figure 1. IHC analysis of 5 Lipoxygenase/ALOX5 using anti-5 Lipoxygenase/ALOX5 antibody (PB0008).
  5 Lipoxygenase/ALOX5 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-5 Lipoxygenase/ALOX5 Antibody (PB0008) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 2. IHC analysis of 5 Lipoxygenase/ALOX5 using anti-5 Lipoxygenase/ALOX5 antibody (PB0008).
  5 Lipoxygenase/ALOX5 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-5 Lipoxygenase/ALOX5 Antibody (PB0008) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 3. IHC analysis of 5 Lipoxygenase/ALOX5 using anti-5 Lipoxygenase/ALOX5 antibody (PB0008).
  5 Lipoxygenase/ALOX5 was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with rabbit anti-5 Lipoxygenase/ALOX5 Antibody (PB0008) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 4. IHC analysis of 5 Lipoxygenase/ALOX5 using anti-5 Lipoxygenase/ALOX5 antibody (PB0008).
  5 Lipoxygenase/ALOX5 was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with rabbit anti-5 Lipoxygenase/ALOX5 Antibody (PB0008) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 5. Western blot analysis of 5 Lipoxygenase/ALOX5 using anti-5 Lipoxygenase/ALOX5 antibody (PB0008). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human SGC whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-5 Lipoxygenase/ALOX5 antigen affinity purified polyclonal antibody (PB0008) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 5 Lipoxygenase/ALOX5 at approximately 78 kDa. The expected band size for 5 Lipoxygenase/ALOX5 is at 78 kDa.

Anti-5 Lipoxygenase/ALOX5 Antibody

筛选器： All IHC

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  Figure 1. IHC analysis of 5 Lipoxygenase/ALOX5 using anti-5 Lipoxygenase/ALOX5 antibody (BA1799-1).
  5 Lipoxygenase/ALOX5 was detected in a paraffin-embedded section of rat lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-5 Lipoxygenase/ALOX5 Antibody (BA1799-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.

Anti-5 Lipoxygenase/ALOX5 Antibody

筛选器： All WB IHC

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  Figure 1. Western blot analysis of 5 Lipoxygenase/ALOX5 using anti-5 Lipoxygenase/ALOX5 antibody (BA2703). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: SW620 whole cell lysates,
  Lane 2: JURKAT whole cell lysates,
  Lane 3: COLO320 whole cell lysates,
  Lane 4: A549 whole cell lysates,
  Lane 5: MCF-7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-5 Lipoxygenase/ALOX5 antigen affinity purified polyclonal antibody (BA2703) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 5 Lipoxygenase/ALOX5 at approximately 78 kDa. The expected band size for 5 Lipoxygenase/ALOX5 is at 78 kDa.

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  Figure 2. IHC analysis of 5 Lipoxygenase/ALOX5 using anti-5 Lipoxygenase/ALOX5 antibody (BA2703).
  5 Lipoxygenase/ALOX5 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-5 Lipoxygenase/ALOX5 Antibody (BA2703) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.

Anti-AADACL1/NCEH1 Antibody

筛选器： All WB FCM ELISA

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  Figure 1. Western blot analysis of anti-AADACL1/NCEH1 antibody (A06478-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human A431 whole cell lysates,
  Lane 2: human PC-3 whole cell lysates,
  Lane 3: human SIHA whole cell lysates,
  Lane 4: human A549 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AADACL1/NCEH1 antigen affinity purified polyclonal antibody (A06478-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AADACL1/NCEH1 at approximately 46 kDa. The expected band size for AADACL1/NCEH1 is at 46,55 kDa.

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  Figure 2. Flow Cytometry analysis of HepG2 cells using anti-AADACL1/NCEH1 antibody (A06478-1).
  Overlay histogram showing HepG2 cells stained with A06478-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-AADACL1/NCEH1 Antibody (A06478-1, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Anti-AAMP Antibody

筛选器： All WB IHC FCM

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  Figure 1. Western blot analysis of AAMP using anti-AAMP antibody (PB9123).
  Lane 1: recombinant human AAMP protein 0.5ng.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AAMP antigen affinity purified polyclonal antibody (PB9123) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AAMP at approximately 47 kDa.

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  Figure 2. Western blot analysis of AAMP using anti-AAMP antibody (PB9123). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: A431 whole cell lysates,
  Lane 2: HELA whole cell lysates,
  Lane 3: HEPG2 whole cell lysates,
  Lane 4: MCF-7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AAMP antigen affinity purified polyclonal antibody (PB9123) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AAMP at approximately 47 kDa. The expected band size for AAMP is at 47 kDa.

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  Figure 3. IHC analysis of AAMP using anti-AAMP antibody (PB9123).
  AAMP was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-AAMP Antibody (PB9123) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 5. Flow cytometry analysis of K562 cell (1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).

Anti-AANAT Antibody

筛选器： All WB IHC ICC/IF

Anti-AANAT Antibody

筛选器： All WB ELISA

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  Figure 1. Western blot analysis of AANAT using anti-AANAT antibody (A03784-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: mouse eye tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AANAT antigen affinity purified polyclonal antibody (A03784-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AANAT at approximately 23 kDa. The expected band size for AANAT is at 23 kDa.

Anti-AASS Antibody

筛选器： All WB IHC ICC/IF FCM ELISA

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  Figure 1. Western blot analysis of AASS using anti-AASS antibody (A07302-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: HepG2 whole cell lysates,
  Lane 2: HEK293 whole cell lysates,
  Lane 3: rat liver tissue lysates,
  Lane 4: rat kidney tissue lysates,
  Lane 5: mouse liver tissue lysates,
  Lane 6: mouse kidney tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AASS antigen affinity purified polyclonal antibody (A07302-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AASS at approximately 102 kDa. The expected band size for AASS is at 102 kDa.

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  Figure 2. IHC analysis of AASS using anti-AASS antibody (A07302-3).
  AASS was detected in a paraffin-embedded section of human kidney cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-AASS Antibody (A07302-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 3. IHC analysis of AASS using anti-AASS antibody (A07302-3).
  AASS was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-AASS Antibody (A07302-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 4. IF analysis of AASS using anti-AASS antibody (A07302-3).
  AASS was detected in an immunocytochemical section of Caco-2 cells. The section was incubated with rabbit anti-AASS Antibody (A07302-3) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Figure 5. Flow Cytometry analysis of Jurkat cells using anti-AASS antibody (A07302-3).
  Overlay histogram showing Jurkat cells stained with A07302-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AASS Antibody (A07302-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Anti-AATF Antibody

筛选器： All WB IHC IF ICC/IF ELISA

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  Figure 1. Western blot analysis of AATF using anti-AATF antibody (A03945-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human 293T whole cell lysates,
  Lane 2: human HepG2 whole cell lysates,
  Lane 3: human Hela whole cell lysates,
  Lane 4: human A549 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AATF antigen affinity purified polyclonal antibody (A03945-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AATF at approximately 95 kDa. The expected band size for AATF is at 63 kDa.

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  Figure 2. IHC analysis of AATF using anti-AATF antibody (A03945-3) .
  AATF was detected in a paraffin-embedded section of mouse cerebellum tissue. The tissue section was incubated with rabbit anti-AATF Antibody (A03945-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 3. IHC analysis of AATF using anti-AATF antibody (A03945-3) .
  AATF was detected in a paraffin-embedded section of mouse cerebellum tissue. The tissue section was incubated with rabbit anti-AATF Antibody (A03945-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 4. IHC analysis of AATF using anti-AATF antibody (A03945-3) .
  AATF was detected in a paraffin-embedded section of mouse cerebellum tissue. The tissue section was incubated with rabbit anti-AATF Antibody (A03945-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 5. IHC analysis of AATF using anti-AATF antibody (A03945-3) .
  AATF was detected in a paraffin-embedded section of mouse cerebellum tissue. The tissue section was incubated with rabbit anti-AATF Antibody (A03945-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 6. IF analysis of AATF using anti-AATF antibody (A03945-3).
  AATF was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-AATF Antibody (A03945-3) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Figure 7. IF analysis of AATF using anti-AATF antibody (A03945-3) and anti-Beta Tubulin antibody (M01857-3).
  AATF was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-AATF Antibody (A03945-3) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody.The section was counterstained with DAPI (Catalog # AR1176) (Blue).