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Figure 1. Western blot analysis of COX4I1 using anti-COX4I1 antibody (A05442-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-COX4I1 antigen affinity purified polyclonal antibody (A05442-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for COX4I1 at approximately 17 kDa. The expected band size for COX4I1 is at 20 kDa.
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Figure 2. IHC analysis of COX4I1 using anti-COX4I1 antibody (A05442-1).
COX4I1 was detected in a paraffin-embedded section of mouse small intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-COX4I1 Antibody (A05442-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
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Figure 3. IHC analysis of COX4I1 using anti-COX4I1 antibody (A05442-1).
COX4I1 was detected in a paraffin-embedded section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-COX4I1 Antibody (A05442-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
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Figure 4. IHC analysis of COX4I1 using anti-COX4I1 antibody (A05442-1).
COX4I1 was detected in a paraffin-embedded section of rat lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-COX4I1 Antibody (A05442-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
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Figure 5. IHC analysis of COX4I1 using anti-COX4I1 antibody (A05442-1).
COX4I1 was detected in a paraffin-embedded section of rat small intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-COX4I1 Antibody (A05442-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
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Figure 6. IHC analysis of COX4I1 using anti-COX4I1 antibody (A05442-1).
COX4I1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-COX4I1 Antibody (A05442-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
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Figure 7. IHC analysis of COX4I1 using anti-COX4I1 antibody (A05442-1).
COX4I1 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-COX4I1 Antibody (A05442-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
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Figure 8. IHC analysis of COX4I1 using anti-COX4I1 antibody (A05442-1).
COX4I1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-COX4I1 Antibody (A05442-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
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Figure 9. IHC analysis of COX4I1 using anti-COX4I1 antibody (A05442-1).
COX4I1 was detected in a paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-COX4I1 Antibody (A05442-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
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Figure 10. IF analysis of COX4I1 using anti-COX4I1 antibody (A05442-1).
COX4I1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-COX4I1 Antibody (A05442-1) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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Figure 11. Flow Cytometry analysis of U937 cells using anti-COX4I1 antibody (A05442-1).
Overlay histogram showing U937 cells stained with A05442-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-COX4I1 Antibody (A05442-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.