Anti-EGFR Antibody

筛选器： All WB IHC ICC/IF FCM ELISA

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  Western blot analysis of anti-EGFR antibody (A00023-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human A431 whole cell lysates,
  Lane 2: human A549 whole cell lysates,
  Lane 3: human U87 whole cell lysates,
  Lane 4: human HELA whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EGFR antigen affinity purified polyclonal antibody (A00023-5) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EGFR at approximately 180 kDa. The expected band size for EGFR is at 134 kDa.

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  IHC analysis of EGFR using anti-EGFR antibody (A00023-5).
  EGFR was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  IF analysis of EGFR using anti-EGFR antibody (A00023-5).
  EGFR was detected in an immunocytochemical section of A431 cells. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Flow Cytometry analysis of A549 cells using anti-EGFR antibody (A00023-5).
  Overlay histogram showing A549 cells stained with A00023-5 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-EGFR Antibody (A00023-5) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of anti-EGFR antibody (A00023-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human A431 whole cell lysates,
  Lane 2: human A549 whole cell lysates,
  Lane 3: human U87 whole cell lysates,
  Lane 4: human HELA whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EGFR antigen affinity purified polyclonal antibody (A00023-5) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EGFR at approximately 180 kDa. The expected band size for EGFR is at 134 kDa.

• 

  IHC analysis of EGFR using anti-EGFR antibody (A00023-5).
  EGFR was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

• 

  IF analysis of EGFR using anti-EGFR antibody (A00023-5).
  EGFR was detected in an immunocytochemical section of A431 cells. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Flow Cytometry analysis of A549 cells using anti-EGFR antibody (A00023-5).
  Overlay histogram showing A549 cells stained with A00023-5 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-EGFR Antibody (A00023-5) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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