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Anti-EGFR Antibody (Clone#OTI2G8)

Mouse monoclonal antibody

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M00023-1

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 5-7天
  • Anti-EGFR mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EGFR .

    all(2)
  • HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EGFR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EGFR.

    all(2)

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产品名称
Anti-EGFR Antibody (Clone#OTI2G8)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
EGFR; ERBB; ERBB1; HER1; mENA; PIG61; Proto oncogene c ErbB 1
产品类型
Monoclonal
检验物种
human, mouse, rat
应用范围
WB, ICC/IF
基因名称
EGFR
克隆号
OTI2G8
宿主
Mouse
抗体亚型
IgG1
免疫原
Full length human recombinant protein of human EGFR(NP_958440) produced in HEK293T cell.
计算分子量
134 kDa
实际分子量
42.4 kDa
成分
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGFalpha). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.
Uniprot ID
P00533  
文献引用格式
EGFR Antibody (Clone#OTI2G8) (Boster Biological Technology, Wuhan, China. Catalog#M00023-1)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
epidermal growth factor receptor
蛋白名全称
Epidermal growth factor receptor
推荐稀释比
Western blot (WB):1:4000
Immunocytochemistry/Immunofluorescence (ICC/IF):1:100

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