Anti-EGFR Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ELISA

A00023-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of EGFR using anti-EGFR antibody (A00023-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates,
Lane 3: mouse testicular tissue lysates,
Lane 4: mouse spleen tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse RAW264.7 whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EGFR antigen affinity purified polyclonal antibody (A00023-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EGFR at approximately 175 kDa. The expected band size for EGFR is at 135 kDa.
all(4)
IHC analysis of EGFR using anti-EGFR antibody (A00023-2).
EGFR was detected in a paraffin-embedded section of mouse liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-EGFR Antibody (A00023-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of EGFR using anti-EGFR antibody (A00023-2).
EGFR was detected in a paraffin-embedded section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-EGFR Antibody (A00023-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
all(4)

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产品简介>

产品名称

Anti-EGFR Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

EGFR; ERBB; ERBB1; HER1; mENA; PIG61; Proto oncogene c ErbB 1

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

WB, IHC, ELISA

基因名称

EGFR

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived mouse EGFR recombinant protein (Position: L25-P596).

计算分子量

135 kDa

实际分子量

175 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is a transmembrane protein that is a receptor for members of the epidermal growth factor family (EGF family) of extracellular protein ligands. It is mapped to 11 A2; 11 9.41 cM. The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.

Uniprot ID

Q01279

RRID

AB_3080889

文献引用格式

EGFR Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00023-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

epidermal growth factor receptor

蛋白名全称

Epidermal growth factor receptor

实验方案>

实验步骤

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