Anti-EGFR Antibody (Clone#AEF-5)

Rabbit monoclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC ICC/IF IP FCM

BM4009

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of anti-EGFR antibody (BM4009). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human U-87 MG whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EGFR antigen affinity purified monoclonal antibody (BM4009) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EGFR at approximately 175 kDa. The expected band size for EGFR is at 134 kDa.
all(5)
all(5)
all(5)
all(5)
all(5)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-EGFR Antibody (Clone#AEF-5)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

EGFR; ERBB; ERBB1; HER1; mENA; PIG61; Proto oncogene c ErbB 1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

EGFR

克隆号

AEF-5

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human EGFR (ErbB 1)

计算分子量

134 kDa

实际分子量

175 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor α (TGFα). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.

Uniprot ID

P00533

文献引用格式

EGFR Antibody (Clone#AEF-5) (Boster Biological Technology, Wuhan, China. Catalog#BM4009)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

实验方案>

实验步骤

引用文献(0)>

清除