| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |

Western blot analysis of F2 using anti-F2 antibody (A00044). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human plasma lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-F2 antigen affinity purified polyclonal antibody (A00044) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for F2 at approximately 70-100 kDa. The expected band size for F2 is at 70 kDa.

IHC analysis of F2 using anti-F2 antibody (A00044).
F2 was detected in a paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-F2 Antibody (A00044) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of F2 using anti-F2 antibody (A00044).
F2 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-F2 Antibody (A00044) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

Flow Cytometry analysis of H-PBMC cells using anti- Prothrombin antibody (A00044).
Overlay histogram showing H-PBMC cells stained with A00044 (Blue line). Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Western blot analysis of F2 using anti-F2 antibody (A00044). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human plasma lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-F2 antigen affinity purified polyclonal antibody (A00044) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for F2 at approximately 70-100 kDa. The expected band size for F2 is at 70 kDa.

IHC analysis of F2 using anti-F2 antibody (A00044).
F2 was detected in a paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-F2 Antibody (A00044) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of F2 using anti-F2 antibody (A00044).
F2 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-F2 Antibody (A00044) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

Flow Cytometry analysis of H-PBMC cells using anti- Prothrombin antibody (A00044).
Overlay histogram showing H-PBMC cells stained with A00044 (Blue line). Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.





