Anti-F2 Antibody (Clone#5B6G1)

Mouse monoclonal antibody

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筛选器： All WB

M00044-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of F2 using anti-F2 antibody (M00044-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: HEPG2 whole cell lysates,
Lane 3: CACO-2 whole cell lysates,
Lane 4: HL-60 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: RH35 whole cell lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-F2 antigen affinity purified monoclonal antibody (M00044-3) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for F2 at approximately 70-100 kDa. The expected band size for F2 is at 70 kDa.
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产品名称

Anti-F2 Antibody (Clone#5B6G1)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

Coagulation factor II; F2; Prothrombin; PT

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

基因名称

克隆号

5B6G1

宿主

Mouse

抗体亚型

IgG1

免疫原

E. coli-derived human Prothrombin recombinant protein (Position: Y97-R124).

计算分子量

70 kDa

实际分子量

70-100 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

F2 (Coagulation Factor II), also known as thrombin, is a serine protease that in humans is encoded by the F2 gene. This gene for human prothrombin (F2) was assigned to chromosome 11p11-q12 by analysis of a panel of somatic cell hybrid DNAs and by in situ hybridization, using both cDNA and genomic probes. The activated thrombin enzyme plays an important role in hemostasis and thrombosis: it converts fibrinogen to fibrin for blood clot formation, stimulates platelet aggregation, and activates coagulation factors V, VIII (F8), and XIII (F13A1). Thrombin also inhibits coagulation by activating protein C.

Uniprot ID

P00734

文献引用格式

F2 Antibody (Clone#5B6G1) (Boster Biological Technology, Wuhan, China. Catalog#M00044-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

coagulation factor II (thrombin)

蛋白名全称

Prothrombin

实验方案>

实验步骤

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