Immunohistochemistry (IHC): | 1:50-400 |
ELISA: | 1:100-1000 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
IHC analysis of CD4 using anti-CD4 antibody (A00344-3).
CD4 was detected in a paraffin-embedded section of rat spleen tissue. The tissue section was incubated with rabbit anti-CD4 Antibody (A00344-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CD4 using anti-CD4 antibody (A00344-3).
CD4 was detected in a paraffin-embedded section of rat spleen tissue. The tissue section was incubated with rabbit anti-CD4 Antibody (A00344-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.