Western blot (WB): | 1:1000~2000 |
Immunohistochemistry (IHC): | 1:50 |
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD4 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4(Cat# MA00344).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD4 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4(Cat# MA00344).