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Anti-CD4 Antibody (Clone#DOE-3)

Rabbit monoclonal antibody

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筛选器: All WB IHC ICC/IF FCM

BM4263

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of anti-CD4 antibody (BM4263). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human THP-1 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD4 antigen affinity purified monoclonal antibody (BM4263) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD4 at approximately 55 kDa. The expected band size for CD4 is at 51 kDa.

    all(9)
  • IHC analysis of CD4 using anti-CD4 antibody (BM4263) .
    CD4 was detected in a paraffin-embedded section of human appendix tissue. The tissue section was incubated with rabbit anti-CD4 Antibody (BM4263) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(9)
  • IHC analysis of CD4 using anti-CD4 antibody (BM4263) .
    CD4 was detected in a paraffin-embedded section of human appendix tissue. The tissue section was incubated with rabbit anti-CD4 Antibody (BM4263) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(9)
  • IHC analysis of CD4 using anti-CD4 antibody (BM4263) .
    CD4 was detected in a paraffin-embedded section of human appendix tissue. The tissue section was incubated with rabbit anti-CD4 Antibody (BM4263) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(9)
  • IHC analysis of CD4 using anti-CD4 antibody (BM4263) .
    CD4 was detected in a paraffin-embedded section of human appendix tissue. The tissue section was incubated with rabbit anti-CD4 Antibody (BM4263) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(9)
  • IHC analysis of CD4 using anti-CD4 antibody (BM4263) .
    CD4 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-CD4 Antibody (BM4263) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(9)
  • IHC analysis of CD4 using anti-CD4 antibody (BM4263) .
    CD4 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-CD4 Antibody (BM4263) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(9)

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产品简介>

产品名称
Anti-CD4 Antibody (Clone#DOE-3)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
CD4; CD4 molecule; CD4mut
产品类型
Monoclonal
检验物种
human
应用范围
WB, IHC, ICC/IF, FCM
基因名称
CD4
克隆号
DOE-3
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthesized peptide derived from human CD4
计算分子量
51 kDa
实际分子量
52 kDa
成分
500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.
纯化方式
Affinity-chromatography
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene.
Uniprot ID
P01730  
文献引用格式
CD4 Antibody (Clone#DOE-3) (Boster Biological Technology, Wuhan, China. Catalog#BM4263)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
T-cell surface glycoprotein CD4
蛋白名全称
T-cell surface glycoprotein CD4
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-200
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-200
Flow Cytometry (FCM):1:40

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    Anti-CD4 Antibody (Clone#DOE-3)

    筛选器: All WB IHC ICC/IF FCM

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