Flow Cytometry analysis of human PBMC cells using anti-CD4 antibody (BM0212).Overlay histogram showing human PBMC cells stained with BM0212 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD4 Antibody (BM0212,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
all(3)
ICC analysis of CD4 using anti-CD4 antibody (BM0212).
CD4 was detected in an immunocytochemical section of human serum. The section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB (Catalog # AR1027) as the chromogen.
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Flow Cytometry analysis of human PBMC cells using anti-CD4 antibody (BM0212).Overlay histogram showing human PBMC cells stained with BM0212 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD4 Antibody (BM0212,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
ICC analysis of CD4 using anti-CD4 antibody (BM0212).
CD4 was detected in an immunocytochemical section of human serum. The section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of human PBMC cells using anti-CD4 antibody (BM0212).Overlay histogram showing human PBMC cells stained with BM0212 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD4 Antibody (BM0212,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
ICC analysis of CD4 using anti-CD4 antibody (BM0212).
CD4 was detected in an immunocytochemical section of human serum. The section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB (Catalog # AR1027) as the chromogen.
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