Anti-Integrin Beta 4/ITGB4 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC IF FCM ELISA

A01015-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Integrin Beta 4/ITGB4 using anti-Integrin Beta 4/ITGB4 antibody (A01015-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human T-47D whole cell lysates,
Lane 4: human HELA whole cell lysates,
Lane 5: human SW620 whole cell lysates,
Lane 6: human CACO-2 whole cell lysates,
Lane 7: Rat ovary tissue lysates,
Lane 8: Mouse ovary tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Integrin Beta 4/ITGB4 antigen affinity purified polyclonal antibody (A01015-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Integrin Beta 4/ITGB4 at approximately 210 kDa. The expected band size for Integrin Beta 4/ITGB4 is at 202 kDa.
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IHC analysis of Integrin Beta 4/ITGB4 using anti-Integrin Beta 4/ITGB4 antibody (A01015-2).
Integrin Beta 4/ITGB4 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Integrin Beta 4/ITGB4 Antibody (A01015-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of Integrin Beta 4/ITGB4 using anti-Integrin Beta 4/ITGB4 antibody (A01015-2).
Integrin Beta 4/ITGB4 was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Integrin Beta 4/ITGB4 Antibody (A01015-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis using anti-ITGB4 antibody (A01015-2) . detected in paraffin-embedded section of human intestinal cancer tissue. The tissue section were stained using the Fluoro488 conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog®#®BA1127) and counterstained with DAPI (blue).
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Flow Cytometry analysis of Raji cells using anti-Integrin Beta 4/ITGB4 antibody (A01015-2).
Overlay histogram showing Raji cells stained with A01015-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Integrin Beta 4/ITGB4 Antibody (A01015-2) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Integrin Beta 4/ITGB4 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

CD104; GP150; Integrin beta 4; integrin; beta 4; ITGB4

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IF, FCM, ELISA

基因名称

ITGB4

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human Integrin beta 4/ITGB4 recombinant protein (Position: R29-K431).

计算分子量

202 kDa

实际分子量

210 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

ITGB4(Integrin, beta-4), also known as CD104 (Cluster of Differentiation 104), is a human gene. The gene encodes the integrin beta 4 subunits, a receptor for the laminins. This subunit tends to associate with alpha 6 subunits and is likely to play a pivotal role in the biology of invasive carcinoma. The ITGB4 gene is mapped on 17q25.1. Using expression profiling, Yang et al. found that ITGB4 was upregulated 6-fold by ZKSCAN3 in transfected human colon cancer cells compared with parental cells. They confirmed that ZKSCAN3 bound the promoter of ITGB4 in vitro and in vivo. ITGB4 knockdown by short hairpin RNA countered ZKSCAN3-augmented anchorage-independent colony formation in the colon cancer cell lines. The integrin beta-4 subunit is characterized by an unusually long cytoplasmic domain that harbors 4 fibronectin type III (FNIII) repeats, residing in 2 pairs separated by a connecting segment. Vidal et al. found compound heterozygosity for mutations in the ITGB4 gene in an infant with junctional epidermolysis bullosa associated with pyloric atresia.

Uniprot ID

P16144

文献引用格式

Integrin Beta 4/ITGB4 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01015-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

integrin, beta 4

蛋白名全称

Integrin beta-4

实验方案>

实验步骤

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