Anti-Integrin Beta 4/ITGB4 Antibody (Clone#AAFE-9)

Rabbit monoclonal antibody

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筛选器： All WB IHC

BM5045

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-Integrin Beta 4/ITGB4 antibody (BM5045). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Integrin Beta 4/ITGB4 antigen affinity purified monoclonal antibody (BM5045) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Integrin Beta 4/ITGB4 at approximately 210 kDa. The expected band size for Integrin Beta 4/ITGB4 is at 202 kDa.
all(2)
IHC analysis of Integrin Beta 4/ITGB4 using anti-Integrin Beta 4/ITGB4 antibody (BM5045).
Integrin Beta 4/ITGB4 was detected in a paraffin-embedded section of human skin cancer tissue. The tissue section was incubated with rabbit anti-Integrin Beta 4/ITGB4 Antibody (BM5045) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(2)

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产品简介>

产品名称

Anti-Integrin Beta 4/ITGB4 Antibody (Clone#AAFE-9)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

CD104; GP150; Integrin beta 4; integrin; beta 4; ITGB4

产品类型

Monoclonal

检验物种

human, mouse

应用范围

WB, IHC

基因名称

ITGB4

克隆号

AAFE-9

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Integrin beta 4

计算分子量

202 kDa

实际分子量

210 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

ITGB4(Integrin, beta-4), also known as CD104 (Cluster of Differentiation 104), is a human gene. The gene encodes the integrin beta 4 subunits, a receptor for the laminins. This subunit tends to associate with alpha 6 subunits and is likely to play a pivotal role in the biology of invasive carcinoma. The ITGB4 gene is mapped on 17q25.1. Using expression profiling, Yang et al. found that ITGB4 was upregulated 6-fold by ZKSCAN3 in transfected human colon cancer cells compared with parental cells. They confirmed that ZKSCAN3 bound the promoter of ITGB4 in vitro and in vivo. ITGB4 knockdown by short hairpin RNA countered ZKSCAN3-augmented anchorage-independent colony formation in the colon cancer cell lines. The integrin beta-4 subunit is characterized by an unusually long cytoplasmic domain that harbors 4 fibronectin type III (FNIII) repeats, residing in 2 pairs separated by a connecting segment. Vidal et al. found compound heterozygosity for mutations in the ITGB4 gene in an infant with junctional epidermolysis bullosa associated with pyloric atresia.

Uniprot ID

P16144

文献引用格式

Integrin Beta 4/ITGB4 Antibody (Clone#AAFE-9) (Boster Biological Technology, Wuhan, China. Catalog#BM5045)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Integrin beta-4

实验方案>

实验步骤

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