Anti-Integrin Beta 4/ITGB4 Antibody (Clone#7G10D2)

筛选器： All WB ICC/IF FCM

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  Western blot analysis of Integrin Beta 4/ITGB4 using anti-Integrin Beta 4/ITGB4 antibody (M01015-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: A549 whole cell lysates,
  Lane 2: PC-3 whole cell lysates,
  Lane 3: Caco-2 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Integrin Beta 4/ITGB4 antigen affinity purified monoclonal antibody (M01015-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Integrin Beta 4/ITGB4 at approximately 210 kDa. The expected band size for Integrin Beta 4/ITGB4 is at 202 kDa.

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  ICC/IF analysis of Integrin Beta 4/ITGB4 using anti-Integrin Beta 4/ITGB4 antibody (M01015-2).
  Integrin Beta 4/ITGB4 was detected in an immunocytochemical section of A431 cells. The section was incubated with mouse anti-Integrin Beta 4/ITGB4 Antibody (M01015-2) at a dilution of 1:100. Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Flow Cytometry analysis of MCF-7 cells using anti-Integrin Beta 4/ITGB4 antibody (M01015-2).
  Overlay histogram showing MCF-7 cells stained with M01015-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Integrin Beta 4/ITGB4 Antibody (M01015-2) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of Integrin Beta 4/ITGB4 using anti-Integrin Beta 4/ITGB4 antibody (M01015-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: A549 whole cell lysates,
  Lane 2: PC-3 whole cell lysates,
  Lane 3: Caco-2 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Integrin Beta 4/ITGB4 antigen affinity purified monoclonal antibody (M01015-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Integrin Beta 4/ITGB4 at approximately 210 kDa. The expected band size for Integrin Beta 4/ITGB4 is at 202 kDa.

• 

  ICC/IF analysis of Integrin Beta 4/ITGB4 using anti-Integrin Beta 4/ITGB4 antibody (M01015-2).
  Integrin Beta 4/ITGB4 was detected in an immunocytochemical section of A431 cells. The section was incubated with mouse anti-Integrin Beta 4/ITGB4 Antibody (M01015-2) at a dilution of 1:100. Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Flow Cytometry analysis of MCF-7 cells using anti-Integrin Beta 4/ITGB4 antibody (M01015-2).
  Overlay histogram showing MCF-7 cells stained with M01015-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Integrin Beta 4/ITGB4 Antibody (M01015-2) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.