Anti-Actin Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

BA0410

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Actin using anti-Actin antibody (BA0410). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: human A431 whole cell lysates,
Lane 6: human U87 whole cell lysates,
Lane 7: human U937 whole cell lysates,
Lane 8: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Actin antigen affinity purified polyclonal antibody (BA0410) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Actin at approximately 42 kDa. The expected band size for Actin is at 42 kDa.
all(8)
Western blot analysis of Actin using anti-Actin antibody (BA0410). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat brain tissue lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: mouse heart tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse spleen tissue lysates,
Lane 7: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Actin antigen affinity purified polyclonal antibody (BA0410) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Actin at approximately 42 kDa. The expected band size for Actin is at 42 kDa.
all(8)
IHC analysis of Actin using anti-Actin antibody (BA0410) .
Actin was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-Actin Antibody (BA0410) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of Actin using anti-Actin antibody (BA0410) .
Actin was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-Actin Antibody (BA0410) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of Actin using anti-Actin antibody (BA0410) .
Actin was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-Actin Antibody (BA0410) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of Actin using anti-Actin antibody (BA0410) .
Actin was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-Actin Antibody (BA0410) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of Actin using anti-Actin antibody (BA0410) .
Actin was detected in a paraffin-embedded section of mouse heart tissue. The tissue section was incubated with rabbit anti-Actin Antibody (BA0410) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of Actin using anti-Actin antibody (BA0410) .
Actin was detected in a paraffin-embedded section of rat heart tissue. The tissue section was incubated with rabbit anti-Actin Antibody (BA0410) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Actin Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Alpha-actin-1; ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; SHPM; CFTD1; CFTDM; CMYP2A; CMYP2B; CMYP2C

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

ACTA1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Actin identical to the related mouse and rat sequences.

计算分子量

42 kDa

实际分子量

42 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.

Uniprot ID

P68133

文献引用格式

Actin Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0410)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Actin, alpha skeletal muscle

实验方案>

实验步骤

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