Anti-Actin/ACTA1 Antibody (Clone#5C5)

Mouse monoclonal antibody

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筛选器： All WB IHC

BM0001

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-Actin/ACTA1 antibody (BM0001). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: monkey heart tissue lysates,
Lane 2: fish heart tissue lysates,
Lane 3: chicken heart tissue lysates,
Lane 4: rat heart tissue lysates,
Lane 5: rat skeletal muscle tissue lysates,
Lane 6: mouse heart tissue lysates,
Lane 7: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Actin/ACTA1 antigen affinity purified monoclonal antibody (BM0001) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Actin/ACTA1 at approximately 42 kDa. The expected band size for Actin/ACTA1 is at 42 kDa.
all(4)
IHC analysis using anti-ACTA1 antibody (BM0001). detected in paraffin-embedded section of human prostatic cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(4)
IHC analysis using anti-ACTA1 antibody (BM0001). detected in paraffin-embedded section of rat cardiac muscle tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(4)
IHC analysis using anti-ACTA1 antibody (BM0001). detected in paraffin-embedded section of mouse cardiac muscle tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(4)

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产品简介>

产品名称

Anti-Actin/ACTA1 Antibody (Clone#5C5)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Alpha-actin-1; ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; SHPM; CFTD1; CFTDM; CMYP2A; CMYP2B; CMYP2C

产品类型

Monoclonal

检验物种

human, mouse, rat, chicken, fish, frog, monkey, rabbit, snake

应用范围

WB, IHC

基因名称

ACTA1

克隆号

5C5

宿主

Mouse

抗体亚型

IgM

免疫原

Polypeptide

计算分子量

42 kDa

实际分子量

42 kDa

成分

200ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

200ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

Uniprot ID

P68133

文献引用格式

Actin/ACTA1 Antibody (Clone#5C5) (Boster Biological Technology, Wuhan, China. Catalog#BM0001)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Actin, alpha skeletal muscle

实验方案>

实验步骤

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