Anti-Actin Antibody (Clone#3H5)

Mouse monoclonal antibody

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筛选器： All WB IHC

M02014-5

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Actin using anti-Actin antibody (M02014-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Human hepg2 whole cell lysates,
Lane 2: Human SKOV3 whole cell lysates,
Lane 3: Human PANC-1 whole cell lysates,
Lane 4: Human HELA whole cell lysates,
Lane 5: Human A549 whole cell lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Actin antigen affinity purified monoclonal antibody (M02014-5) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Actin at approximately 42 kDa. The expected band size for Actin is at 42 kDa.
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IHC analysis of Actin using anti-Actin antibody (M02014-5).
Actin was detected in a paraffin-embedded section of human bladder cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Actin Antibody (M02014-5) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(2)

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产品名称

Anti-Actin Antibody (Clone#3H5)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Alpha-actin-1; ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; SHPM; CFTD1; CFTDM; CMYP2A; CMYP2B; CMYP2C

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

ACTA1

克隆号

3H5

宿主

Mouse

抗体亚型

IgG1

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Actin, identical to the related mouse and rat sequences.

计算分子量

42 kDa

实际分子量

42 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.

Uniprot ID

P68133

RRID

AB_3081860

文献引用格式

Actin Antibody (Clone#3H5) (Boster Biological Technology, Wuhan, China. Catalog#M02014-5)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

actin, alpha 1, skeletal muscle

蛋白名全称

Actin, alpha skeletal muscle

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