Anti-Actin/ACTA1 Antibody (Clone#C-1)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP FCM

BM4047

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Actin (Alpha-Actin) expression in (1) A431 cell lysate; (2) MCF-7 cell lysate.
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IHC analysis of ACTA1 using anti-ACTA1 antibody (BM4047) .
ACTA1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-ACTA1 Antibody (BM4047) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of ACTA1 using anti-ACTA1 antibody (BM4047) .
ACTA1 was detected in a paraffin-embedded section of human prostate cancer tissue. The tissue section was incubated with rabbit anti-ACTA1 Antibody (BM4047) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of ACTA1 using anti-ACTA1 antibody (BM4047) .
ACTA1 was detected in a paraffin-embedded section of mouse heart tissue. The tissue section was incubated with rabbit anti-ACTA1 Antibody (BM4047) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of ACTA1 using anti-ACTA1 antibody (BM4047) .
ACTA1 was detected in a paraffin-embedded section of rat heart tissue. The tissue section was incubated with rabbit anti-ACTA1 Antibody (BM4047) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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Immunofluorescent analysis of Hela cells, using Actin (Alpha-Actin) Antibody .
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产品简介>

产品名称

Anti-Actin/ACTA1 Antibody (Clone#C-1)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Alpha-actin-1; ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; SHPM; CFTD1; CFTDM; CMYP2A; CMYP2B; CMYP2C

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

ACTA1

克隆号

C-1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Actin (Alpha-Actin)

计算分子量

42 kDa

实际分子量

42 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.

Uniprot ID

P68133

文献引用格式

Actin/ACTA1 Antibody (Clone#C-1) (Boster Biological Technology, Wuhan, China. Catalog#BM4047)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Actin, alpha skeletal muscle

实验方案>

实验步骤

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