Anti-Caspase 3/CASP3 (p12) Antibody

Rabbit polyclonal antibody

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筛选器： All WB

BA2142

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of Caspase 3/CASP3 (p12) using anti-Caspase 3/CASP3 (p12) antibody (BA2142). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat cardiac muscle tissue lysates,
Lane 2: Rat liver tissue lysates,
Lane 3: Rat thymus tissue lysates,
Lane 4: MCF-7 whole cell lysates,
Lane 5: SMMC whole cell lysates,
Lane 6: HT1080 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Caspase 3/CASP3 (p12) antigen affinity purified polyclonal antibody (BA2142) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Caspase 3/CASP3 (p12) at approximately 35 kDa,(cleaved)20/17/12 kDa. The expected band size for Caspase 3/CASP3 (p12) is at 32 kDa.
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产品名称

Anti-Caspase 3/CASP3 (p12) Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Apopain; CASP 3; CASP3; Caspase 3; Caspase 3/p17; Caspase3; CPP 32; CPP32; CPP32B; Cysteine protease CPP32; Protein Yama; SCA 1; SREBP cleavage activity 1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

CASP3

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminal of human Caspase 3, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

计算分子量

32 kDa

实际分子量

35 kDa,(cleaved)20/17/12 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Caspase 3(caspase 3, apoptosis-related cysteine peptidase) is a caspase protein that interacts with caspase 8 and caspase 9, also known as Caspase-3, PARP CLEAVAGE PROTEASE, APOPAIN, CPP32, CPP32B, YAMA. It is a member of the cysteine-aspartic acid protease(caspase) family. PCR analysis of 16 human tissues revealed expression of full-length CASP3, as well as CASP3s at somewhat lower levels, in all tissues tested. Western blot analysis of 3 cell lines revealed the prominent CASP3 band at 32 kD and CASP3s at 20 kD. Several human cancer cell lines showed coexpression of both variants at the mRNA and protein levels. Overexpression of the catalytically inactive CASP3s by human kidney cells offered some resistance to inducers of apoptosis, and CASP3s accumulation could be enhanced with addition of proteasome inhibitors. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Alternative splicing of this gene results in two transcript variants that encode the same protein. Encoded by the CASP3 gene, CASP3 orthologs have been identified in numerous mammals for which complete genome data are available. Unique orthologs are also present in birds, lizards, lissamphibians, and teleosts. Nicholson et al. developed a potent peptide aldehyde inhibitor and showed that it prevented apoptotic events in vitro, suggesting that apopain/CPP32 is important for the initiation of apoptotic cell death.

Uniprot ID

P42574

文献引用格式

Caspase 3/CASP3 (p12) Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA2142)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

caspase 3, apoptosis-related cysteine peptidase

蛋白名全称

Caspase-3(CASP-3)

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