Anti-Caspase 3/CASP3 (p17) Antibody

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC ICC/IF

PB9188

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Figure 1. Western blot analysis of Caspase 3/CASP3 (p17) using anti-Caspase 3/CASP3 (p17) antibody (PB9188). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: rat thymus tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse NIH/3T3 whole cell lysates,
Lane 6: mouse RAW264.7 whole cell lysates,
Lane 7: mouse ANA-1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Caspase 3/CASP3 (p17) antigen affinity purified polyclonal antibody (PB9188) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Caspase 3/CASP3 (p17) at approximately 35 kDa,(cleaved)20/17/12 kDa. The expected band size for Caspase 3/CASP3 (p17) is at 32 kDa.
all(4)
Figure 2. Western blot analysis of Caspase 3/CASP3 (p17) using anti-Caspase 3/CASP3 (p17) antibody (PB9188). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Caspase 3/CASP3 (p17) antigen affinity purified polyclonal antibody (PB9188) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Caspase 3/CASP3 (p17) at approximately 35 kDa,(cleaved)20/17/12 kDa. The expected band size for Caspase 3/CASP3 (p17) is at 32 kDa.
all(4)
Figure 3. IHC analysis of Caspase 3/CASP3 (p17) using anti-Caspase 3/CASP3 (p17) antibody (PB9188).
Caspase 3/CASP3 (p17) was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-Caspase 3/CASP3 (p17) Antibody (PB9188) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
all(4)
Figure 4. IF analysis of Caspase 3/CASP3 (p17) using anti-Caspase 3/CASP3 (p17) antibody (PB9188).
Caspase 3/CASP3 (p17) was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-Caspase 3/CASP3 (p17) Antibody (PB9188) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody.
all(4)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Caspase 3/CASP3 (p17) Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Apopain; CASP 3; CASP3; Caspase 3; Caspase 3/p17; Caspase3; CPP 32; CPP32; CPP32B; Cysteine protease CPP32; Protein Yama; SCA 1; SREBP cleavage activity 1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

CASP3

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human Caspase 3 recombinant protein (Position: T67-D175). Human Caspase 3 shares 86% and 90% amino acid (aa) sequences identity with mouse and rat Caspase 3, respectively.

计算分子量

32 kDa

实际分子量

35 kDa,(cleaved)20/17/12 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Caspase 3 is a caspase protein which interacts with Survivin, XIAP, CFLAR, Caspase 8, HCLS1, Deleted in Colorectal Cancer, TRAF3 and GroEL. This gene which is located on 4q35 encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. And the caspase-3 activation in heart failure sequentially cleaves SRF and generates a truncated SRF that appears to function as a dominant-negative transcription factor. Additionally, the caspase-3 influence on bone mineral density should be considered in any in vivo application of caspase-3 inhibitors to the treatment of human disease. In erythroid precursors undergoing terminal differentiation, Hsp70 prevents active CASP3 from cleaving GATA1 and inducing apoptosis.

Uniprot ID

P42574

RRID

AB_2890203

文献引用格式

Caspase 3/CASP3 (p17) Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9188)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

caspase 3, apoptosis-related cysteine peptidase

蛋白名全称

Caspase-3

实验方案>

实验步骤

引用文献(0)>

清除