Anti-Caspase 3/CASP3 Antibody (Clone#30C96)

Rabbit monoclonal antibody

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筛选器： All WB IHC IP

M00334-9

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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all(4)
Figure 1. Western blot analysis of anti-Caspase 1/CASP1 antibody (M00334-9). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: human HepG2 whole cell lysates,
Lane 6: human 293T whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Caspase 1/CASP1 antigen affinity purified monoclonal antibody (M00334-9) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Caspase 1/CASP1 at approximately 32 kDa. The expected band size for Caspase 1/CASP1 is at 32 kDa.
all(4)
Figure 2. IHC analysis of Caspase 3/CASP3 using anti-Caspase 3/CASP3 antibody (M00334-9) .
Caspase 3/CASP3 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-Caspase 3/CASP3 Antibody (M00334-9) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
Figure 3. IHC analysis of Caspase 3/CASP3 using anti-Caspase 3/CASP3 antibody (M00334-9) .
Caspase 3/CASP3 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-Caspase 3/CASP3 Antibody (M00334-9) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
all(4)
Figure 1. Western blot analysis of anti-Caspase 1/CASP1 antibody (M00334-9). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: human HepG2 whole cell lysates,
Lane 6: human 293T whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Caspase 1/CASP1 antigen affinity purified monoclonal antibody (M00334-9) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Caspase 1/CASP1 at approximately 32 kDa. The expected band size for Caspase 1/CASP1 is at 32 kDa.
all(4)

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产品简介>

产品名称

Anti-Caspase 3/CASP3 Antibody (Clone#30C96)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Apopain; CASP 3; CASP3; Caspase 3; Caspase 3/p17; Caspase3; CPP 32; CPP32; CPP32B; Cysteine protease CPP32; Protein Yama; SCA 1; SREBP cleavage activity 1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IP

基因名称

CASP3

克隆号

30C96

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Caspase 3

计算分子量

32 kDa

实际分子量

35 kDa,(cleaved)20/17/12 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

Uniprot ID

P42574

文献引用格式

Caspase 3/CASP3 Antibody (Clone#30C96) (Boster Biological Technology, Wuhan, China. Catalog#M00334-9)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

实验方案>

实验步骤

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